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Literature summary extracted from

  • Schoner, R.; Herrmann, K.M.
    3-Deoxy-D-arabino-heptulosonate 7-phosphate synthase. Purification, properties, and kinetics of the tyrosine-sensitive isoenzyme from Escherichia coli (1976), J. Biol. Chem., 251, 5440-5447.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.5.1.54 phosphoenolpyruvate stabilizes Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.54 phosphate non-competitive with respect to both phosphoenolpyruvate and D-erythrose 4-phosphate Escherichia coli
2.5.1.54 Tyr feedback inhibition; noncompetitive with respect to D-erythrose 4-phosphate, competitive with respect to phosphoenolpyruvate; Tyr-sensitive isozyme Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.54 additional information
-
 additional information kinetics Escherichia coli
2.5.1.54 0.0058
-
 phosphoenolpyruvate pH 7.0, 37°C Escherichia coli
2.5.1.54 0.0965
-
 D-erythrose 4-phosphate pH 7.0, 37°C Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.54 39000
-
 x * 39000, either a rapid monomer-dimer equilibrium or a very asymetric shape for the native enzyme, SDS-PAGE Escherichia coli
2.5.1.54 66000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Escherichia coli
-
 tyrosine-sensitive isozyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.54 105fold Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate mechanism Escherichia coli
a
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate ordered ping-pong bi-bi mechanism Escherichia coli
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.54 56
-
 purified enzyme Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.5.1.54 -20°C, in phosphate buffer containing phosphoenolpyruvate, stable for several months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Escherichia coli 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.54 ? x * 39000, either a rapid monomer-dimer equilibrium or a very asymetric shape for the native enzyme, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.54 37
-
 assay at Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.54 25
-
 2 h, in 3(N-morpholono)propane-sulfonic acid buffer, 50% loss of activity Escherichia coli
2.5.1.54 25
-
 1 h, in 1,3-bis[tris(hydroxymethyl)methylamino]propane buffer, 10% loss of activity Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.54 122
-
 phosphoenolpyruvate pH 7.0, 37°C, MW 79000 assumed Escherichia coli
2.5.1.54 122
-
 D-erythrose 4-phosphate pH 7.0, 37°C, MW 79000 assumed Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.54 7
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.54 5.7 8.5 pH 5.7: about 25% of maximal activity, pH 8.5: about 30% of maximal activity Escherichia coli