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Literature summary extracted from

  • Dusha, I.; Dénes, G.
    Purification and properties of tyrosine-sensitive 3-deoxy-D-arabino-heptulosonate-7-phosphate synthetase of Escherichia coli K12 (1976), Biochim. Biophys. Acta, 438, 563-573.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.5.1.54 Co2+ stabilizes Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.54 EDTA reversible by Co2+ Escherichia coli
2.5.1.54 Phe no inhibition Escherichia coli
2.5.1.54 Tyr 0.02 mM, 50% inhibition; Tyr-sensitive isozyme Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.54 additional information
-
 additional information kinetics Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.54 Co2+ 2-2.5fold increase in activity at 0.1 mM Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O Escherichia coli first enzyme of the aromatic amino acid biosynthesis 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Escherichia coli
-
 tyrosine-sensitive isozyme
-
2.5.1.54 Escherichia coli
-
 deletion mutant strain H80c from strain HfrH
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.54
-
 Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate mechanism Escherichia coli
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.54 2.1
-
 purified enzyme Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Escherichia coli 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O first enzyme of the aromatic amino acid biosynthesis Escherichia coli 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.54 24
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.54 7
-
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.54 0.02
-
 Tyr pH 7.0, 24°C Escherichia coli