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Literature summary extracted from

  • Srinivasan, P.R.; Sprinson, D.B.
    2-Keto-3-deoxy-D-arabo-heptonic acid 7-phosphate synthetase (1954), J. Biol. Chem., 234, 716-722.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.54 2-mercaptoethanol activates Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.54 3-deoxy-D-arabino-heptonic acid 7-phosphate
-
 Escherichia coli
2.5.1.54 D-fructose 1,6-diphosphate
-
 Escherichia coli
2.5.1.54 D-sedoheptulose 1,7-diphosphate
-
 Escherichia coli
2.5.1.54 D-sedoheptulose 7-phosphate
-
 Escherichia coli
2.5.1.54 p-chloromercuribenzoate complete inhibition at 0.02 mM, reversible by cysteine Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.54 1.2
-
 D-erythrose 4-phosphate pH 6.4, 37°C Escherichia coli
2.5.1.54 3.5
-
 phosphoenolpyruvate pH 6.4, 37°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Escherichia coli
-
 mutant 83-24
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.54 partial Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate mechanism Escherichia coli
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.54 0.4
-
 partially purified enzyme Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.5.1.54 -15°C, ammonium sulfate and acetone fractions, stable for at least several weeks Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 additional information no activity with D-erythrose, D-glyceraldehyde 3-phosphate, ribose 5-phosphate, glucose 6-phosphate, glucosamine 6-phosphate, N-acetylglucosamine 6-phosphate, and pyruvate Escherichia coli ?
-
 ?
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Escherichia coli 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ir

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.54 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.54 6.4
-
-
 Escherichia coli