Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Higuchi, K.; Kanazawa, K.; Nishizawa, N.K.; Chino, M.; Mori, S.
    Purification and characterization of nicotianamine synthase from Fe-deficient barley roots (1994), Plant Soil, 165, 173-179.
No PubMed abstract available

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.43 30000
-
 x * 30000, SDS-PAGE Hordeum vulgare
2.5.1.43 40000 50000 gel filtration Hordeum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.43 S-adenosyl-L-methionine Hordeum vulgare synthesis of nicotianamide, the key precursor of the mugineic acid family phytosiderophores ?
-
 ?
2.5.1.43 S-adenosyl-L-methionine Hordeum vulgare strongly induced by Fe-deficiency treatment ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.43 Hordeum vulgare
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.43
-
 Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.43 root primary roots Hordeum vulgare
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.43 additional information
-
-
 Hordeum vulgare

Storage Stability

EC Number Storage Stability Organism
2.5.1.43 4°C, in 50 mM Tris, 1 mM EDTA, 3 mM dithiothreitol, pH 8.7 with HCl, stable for about 1 week Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.43 S-adenosyl-L-methionine
-
 Hordeum vulgare 5'-S-methyl-5'-thioadenosine + nicotianamine
-
 ?
2.5.1.43 S-adenosyl-L-methionine synthesis of nicotianamide, the key precursor of the mugineic acid family phytosiderophores Hordeum vulgare ?
-
 ?
2.5.1.43 S-adenosyl-L-methionine strongly induced by Fe-deficiency treatment Hordeum vulgare ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.43 ? x * 30000, SDS-PAGE Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.43 9
-
-
 Hordeum vulgare

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.5.1.43 4
-
 overnight in 50 mM Tris, 1 mM EDTA, 3 mM dithiothreitol, pH 8.7 with HCl, 90% loss of activity Hordeum vulgare
2.5.1.43 5.5
-
 overnight in 50 mM Tris, 1 mM EDTA, 3 mM dithiothreitol, pH 8.7 with HCl, 80% loss of activity Hordeum vulgare
2.5.1.43 7
-
 activity is almost completely recovered after overnight exposure in 50 mM Tris, 1 mM EDTA, 3 mM dithiothreitol, pH 8.7 with HCl Hordeum vulgare