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Literature summary extracted from

  • Higuchi, K.; Kanazawa, K.; Nishizawa, N.K.; Mori, S.
    The role of nicotianamine synthase in response to Fe nutrition status in Gramineae (1996), Plant Soil, 178, 171-177.
No PubMed abstract available

Application

EC Number Application Comment Organism
2.5.1.43 agriculture the nicotianamine synthase gene may be a suitable candidate for making a transgenic plant tolerant to Fe-deficiency Hordeum vulgare

General Stability

EC Number General Stability Organism
2.5.1.43 thiol proteases such as papain might digest nicotianamide synthase in crude extracts Hordeum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.43 S-adenosyl-L-methionine Hordeum vulgare enzyme activity is induced at the 3rd day after withholding Fe supply and declines within one day following the supply of Fe3+-epihydroxymugineic acid ?
-
 ?
2.5.1.43 S-adenosyl-L-methionine Hordeum vulgare enzyme in the biosynthetic pathway of mugineic acids ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.43 Hordeum vulgare
-
-
-
2.5.1.43 Oryza sativa
-
-
-
2.5.1.43 Zea mays
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.43 root
-
 Zea mays
-
2.5.1.43 root
-
 Oryza sativa
-
2.5.1.43 root enzyme activity is induced at the 3rd day after withholding Fe supply and decline within one day following the supply of Fe3+-epihydroxymugineic acid Hordeum vulgare
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.43 S-adenosyl-L-methionine
-
 Hordeum vulgare 5'-S-methyl-5'-thioadenosine + nicotianamine
-
 ?
2.5.1.43 S-adenosyl-L-methionine
-
 Zea mays 5'-S-methyl-5'-thioadenosine + nicotianamine
-
 ?
2.5.1.43 S-adenosyl-L-methionine
-
 Oryza sativa 5'-S-methyl-5'-thioadenosine + nicotianamine
-
 ?
2.5.1.43 S-adenosyl-L-methionine enzyme activity is induced at the 3rd day after withholding Fe supply and declines within one day following the supply of Fe3+-epihydroxymugineic acid Hordeum vulgare ?
-
 ?
2.5.1.43 S-adenosyl-L-methionine enzyme in the biosynthetic pathway of mugineic acids Hordeum vulgare ?
-
 ?

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.43 7.5 10 pH 7.5: about 20% of maximal activity, pH 10.0: about 50% of maximal activity Hordeum vulgare