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Literature summary extracted from

  • Korolev, S.; Ikeguchi, Y.; Skarina T.; Beasley, S.; Arrowsmith, C.; Edwards, A.; Joachimiak, A.; Pegg, A.E.; Savchenko, A.
    The crystal structure of spermidine synthase with a multisubstrate adduct inhibitor (2002), Nat. Struct. Biol., 9, 27-31.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.16 hanging drop vapour diffusion, multiwavelength anomalous diffraction from selenomethionine containing crystals Thermotoga maritima

General Stability

EC Number General Stability Organism
2.5.1.16 tetramerization stabilizes Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.16 S-Adenosyl-1,8-diamino-3-thiooctane IC50 of 0.0002 mM Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.16 0.02
-
 1,4-diaminobutane pH 7.5, 37ºC Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.16 131000
-
 gel filtration Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.16 S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane Thermotoga maritima
-
 5'-methylthioadenosine + spermidine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.16 Thermotoga maritima
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.16 S-adenosyl-(5')-3-methylthio-1-propylamine + 1,3-diaminopropane weak substrate Thermotoga maritima ?
-
 ?
2.5.1.16 S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane
-
 Thermotoga maritima 5'-methylthioadenosine + spermidine
-
 ?
2.5.1.16 S-adenosyl-(5')-3-methylthio-1-propylamine + spermidine weak substrate Thermotoga maritima 5'-methylthioadenosine + spermine
-
 ?
2.5.1.16 S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane
-
 Thermotoga maritima 5'-methylthioadenosine + spermidine
-
 ?
2.5.1.16 S-adenosyl-3-methylthio-1-propylamine + 1,5-diaminopentane weak substrate Thermotoga maritima 5'-methylthioadenosine + N-(3-aminopropyl)-1,5-diaminopentane
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.16 tetramer HPLC size-exclusion chromatography Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
2.5.1.16 PAPT
-
-

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.16 90
-
 more active than at 37ºC Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.16 7.5
-
-
 Thermotoga maritima

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.5.1.16 0.0002
-
 IC50 of 0.0002 mM Thermotoga maritima S-Adenosyl-1,8-diamino-3-thiooctane