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Literature summary extracted from

  • Yoon, S.O.; Lee, Y.S.; Lee, S.H.; Cho, Y.D.
    Polyamine synthesis in plants: isolation and characterization of spermidine synthase from soybean (Glycine max) axes (2000), Biochim. Biophys. Acta, 1475, 17-26.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.5.1.16 freeze-thawing inactivates Glycine max
2.5.1.16 S-adenosyl-(5')-3-methylthio-1-propylamine protects from inhibitors when preincubated Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.16 DTNB potent inhibition, activity is restored to more than 95% with 0.1 mM DTT Glycine max
2.5.1.16 N-ethylmaleimide potent inhibition Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.16 0.0004
-
 S-adenosyl-(5')-3-methylthio-1-propylamine pH 8.0, 37ºC Glycine max
2.5.1.16 0.0325
-
 1,4-diaminobutane pH 8.0, 37ºC Glycine max

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.16 cytosol
-
 Glycine max 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.16 Ca2+ 0.01 mM, activity increases by 200% Glycine max
2.5.1.16 Co2+ 0.01 mM, activity increases by 352% Glycine max
2.5.1.16 Cu2+ 0.01 mM, activity increases by 230% Glycine max
2.5.1.16 K+ not effective Glycine max
2.5.1.16 additional information Li+, Mn2+ and Zn2+, not effective Glycine max
2.5.1.16 Na+ not effective Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.16 74000
-
 gel filtration and SDS-PAGE Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.16 S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane Glycine max
-
 5'-methylthioadenosine + spermidine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.16 Glycine max
-
 soybean
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.16 ammonium sulfate fractionation, chromatography on DEAE-Sephacel, chromatography on Sephacryl S-300, chromatography on aminooctyl-Sepharose and ATPA-Sepharose, 319fold purified Glycine max

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.16 S-adenosyl 3-(methylsulfanyl)propylamine + putrescine = S-methyl-5'-thioadenosine + spermidine uni uni uni uni ping pong mechanism Glycine max
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.16 stem 3 days old Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.16 0.076
-
-
 Glycine max

Storage Stability

EC Number Storage Stability Organism
2.5.1.16 0-4ºC, 10% loss of activity after several months Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.16 S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane
-
 Glycine max 5'-methylthioadenosine + spermidine + H+ ?
2.5.1.16 S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane
-
 Glycine max 5'-methylthioadenosine + spermidine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.16 monomer 1 * 74000, gel filtration and SDS-PAGE Glycine max

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.16 25
-
 stable over 30 min Glycine max
2.5.1.16 37
-
 stable over 30 min Glycine max
2.5.1.16 50
-
 95% loss of activity over 30 min Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.16 8.5
-
 activity is 20% higher in Tris-HCl buffer than in phosphate buffer at the same pH Glycine max