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Literature summary extracted from

  • Laster, L.; Blair, A.
    An intestinal phosphorylase for uric ribonucleoside (1963), J. Biol. Chem., 238, 3348-3357.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.16 1-Ribosyluracil 1 mM, about 85% inhibition Canis lupus familiaris
2.4.2.16 2-thio-6-oxopurine 1 mM, 73% inhibition Canis lupus familiaris
2.4.2.16 2-Thiouracil 1 mM, 96% inhibition Canis lupus familiaris
2.4.2.16 4-amino-5-imidazolecarboxamide 1 mM, about 15% inhibition Canis lupus familiaris
2.4.2.16 5-methyluracil 1 mM, about 90% inhibition Canis lupus familiaris
2.4.2.16 5-methyluridine 1 mM, about 95% inhibition Canis lupus familiaris
2.4.2.16 Colchicine 1 mM, 43% inhibition, competitive Canis lupus familiaris
2.4.2.16 guanosine 1 mM, about 20% inhibition Canis lupus familiaris
2.4.2.16 Inosine 1 mM, about 25% inhibition Canis lupus familiaris
2.4.2.16 p-chloromercuribenzoate reversed by cysteine Canis lupus familiaris
2.4.2.16 Phenylbutazone 1 mM, 30% inhibition, competitive Canis lupus familiaris
2.4.2.16 Uracil 1 mM, about 85% inhibition Canis lupus familiaris
2.4.2.16 xanthine 1 mM, 35% inhibition Canis lupus familiaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.16 uric acid ribonucleoside + phosphate Cavia porcellus
-
 urate + D-ribose 1-phosphate
-
 ?
2.4.2.16 uric acid ribonucleoside + phosphate Rattus norvegicus
-
 urate + D-ribose 1-phosphate
-
 ?
2.4.2.16 uric acid ribonucleoside + phosphate Pigeon
-
 urate + D-ribose 1-phosphate
-
 ?
2.4.2.16 uric acid ribonucleoside + phosphate Canis lupus familiaris
-
 urate + D-ribose 1-phosphate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.16 Canis lupus familiaris
-
-
-
2.4.2.16 Cavia porcellus
-
-
-
2.4.2.16 Pigeon
-
-
-
2.4.2.16 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.16
-
 Canis lupus familiaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.16 heart
-
 Cavia porcellus
-
2.4.2.16 heart
-
 Rattus norvegicus
-
2.4.2.16 heart
-
 Pigeon
-
2.4.2.16 kidney
-
 Cavia porcellus
-
2.4.2.16 kidney
-
 Rattus norvegicus
-
2.4.2.16 kidney
-
 Pigeon
-
2.4.2.16 kidney
-
 Canis lupus familiaris
-
2.4.2.16 liver
-
 Rattus norvegicus
-
2.4.2.16 liver
-
 Pigeon
-
2.4.2.16 skeletal muscle
-
 Rattus norvegicus
-
2.4.2.16 small intestine mucosa
-
 Rattus norvegicus
-
2.4.2.16 small intestine mucosa
-
 Pigeon
-
2.4.2.16 small intestine mucosa
-
 Canis lupus familiaris
-
2.4.2.16 spleen low activity Rattus norvegicus
-
2.4.2.16 testis
-
 Rattus norvegicus
-
2.4.2.16 testis
-
 Pigeon
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.16 4.6
-
-
 Canis lupus familiaris

Storage Stability

EC Number Storage Stability Organism
2.4.2.16 -10°C, partially purified enzyme is stable for at least 2 months Canis lupus familiaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.16 uric acid ribonucleoside + arsenate
-
 Cavia porcellus urate + D-ribose 1-phosphate
-
 ?
2.4.2.16 uric acid ribonucleoside + arsenate
-
 Rattus norvegicus urate + D-ribose 1-phosphate
-
 ?
2.4.2.16 uric acid ribonucleoside + arsenate
-
 Pigeon urate + D-ribose 1-phosphate
-
 ?
2.4.2.16 uric acid ribonucleoside + arsenate reverse reaction not demonstrated, arsenate can replace phosphate at a lower reaction rate Canis lupus familiaris urate + D-ribose 1-phosphate
-
 ir
2.4.2.16 uric acid ribonucleoside + phosphate
-
 Cavia porcellus urate + D-ribose 1-phosphate
-
 ?
2.4.2.16 uric acid ribonucleoside + phosphate
-
 Rattus norvegicus urate + D-ribose 1-phosphate
-
 ?
2.4.2.16 uric acid ribonucleoside + phosphate
-
 Pigeon urate + D-ribose 1-phosphate
-
 ?
2.4.2.16 uric acid ribonucleoside + phosphate
-
 Canis lupus familiaris urate + D-ribose 1-phosphate
-
 ir

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.16 50
-
 pH 7.8, 5 min, 5% loss of activity, partially purified enzyme Canis lupus familiaris
2.4.2.16 60
-
 pH 7.8, 5 min, 40% loss of activity, partially purified enzyme Canis lupus familiaris
2.4.2.16 70
-
 pH 7.8, 5 min, 95% loss of activity, partially purified enzyme Canis lupus familiaris
2.4.2.16 80
-
 pH 7.8, 5 min, 100% loss of activity, partially purified enzyme Canis lupus familiaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.16 7.5 8 reaction with phosphate or arsenate Canis lupus familiaris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.2.16 6 9.5 pH 6.0: about 60% of maximal activity, pH 9.5: 45% of maximal activity Canis lupus familiaris

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.2.16 4.5
-
 0°C, 10 min, 30% loss of activity, partially purified enzyme Canis lupus familiaris

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.16 1.23
-
 Colchicine 37°C, pH 8.2 Canis lupus familiaris
2.4.2.16 1.76
-
 Phenylbutazone 37°C, pH 8.2 Canis lupus familiaris