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Literature summary extracted from

  • Parham, R.; Rebeiz, C.A.
    Chloroplast biogenesis 72: a [4-vinyl]chlorophyllide a reductase assay using divinyl chlorophyllide a as an exogenous substrate (1995), Anal. Biochem., 231, 164-169.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.1.75 chloroplast
-
 Hordeum vulgare 9507
-
1.3.1.75 chloroplast
-
 Zea mays 9507
-
1.3.1.75 chloroplast
-
 Cucumis sativus 9507
-
1.3.1.75 etioplast
-
 Hordeum vulgare 9513
-
1.3.1.75 etioplast
-
 Zea mays 9513
-
1.3.1.75 etioplast
-
 Cucumis sativus 9513
-
1.3.1.75 membrane
-
 Hordeum vulgare 16020
-
1.3.1.75 membrane
-
 Zea mays 16020
-
1.3.1.75 membrane
-
 Cucumis sativus 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.75 2,4-divinyl chlorophyllide a + NADPH + H+ Hordeum vulgare
-
 2-vinyl-4-ethyl chlorophyllide a + NADP+ i.e. monovinyl chlorophyllide a r
1.3.1.75 2,4-divinyl chlorophyllide a + NADPH + H+ Zea mays
-
 2-vinyl-4-ethyl chlorophyllide a + NADP+ i.e. monovinyl chlorophyllide a r
1.3.1.75 2,4-divinyl chlorophyllide a + NADPH + H+ Cucumis sativus
-
 2-vinyl-4-ethyl chlorophyllide a + NADP+ i.e. monovinyl chlorophyllide a r

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.75 Cucumis sativus
-
 light-inducible
-
1.3.1.75 Cucumis sativus
-
 Beit Alpha MR
-
1.3.1.75 Hordeum vulgare
-
 light-inducible
-
1.3.1.75 Zea mays
-
 light-inducible
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.75 cotyledon etiolated Hordeum vulgare
-
1.3.1.75 cotyledon etiolated Zea mays
-
1.3.1.75 cotyledon etiolated Cucumis sativus
-
1.3.1.75 leaf etiolated Hordeum vulgare
-
1.3.1.75 leaf etiolated Zea mays
-
1.3.1.75 leaf etiolated Cucumis sativus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.75 additional information
-
 assay development Cucumis sativus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.75 2,4-divinyl chlorophyllide a + NADPH + H+
-
 Hordeum vulgare 2-vinyl-4-ethyl chlorophyllide a + NADP+ i.e. monovinyl chlorophyllide a r
1.3.1.75 2,4-divinyl chlorophyllide a + NADPH + H+
-
 Zea mays 2-vinyl-4-ethyl chlorophyllide a + NADP+ i.e. monovinyl chlorophyllide a r
1.3.1.75 2,4-divinyl chlorophyllide a + NADPH + H+
-
 Cucumis sativus 2-vinyl-4-ethyl chlorophyllide a + NADP+ i.e. monovinyl chlorophyllide a r
1.3.1.75 2,4-divinyl chlorophyllide a + NADPH + H+ strictly dependent on divinyl chlorophyllide a and NADPH, no activity with divinyl protochlorophyllide a, NADH and GSH Hordeum vulgare 2-vinyl-4-ethyl chlorophyllide a + NADP+ i.e. monovinyl chlorophyllide a r
1.3.1.75 2,4-divinyl chlorophyllide a + NADPH + H+ strictly dependent on divinyl chlorophyllide a and NADPH, no activity with divinyl protochlorophyllide a, NADH and GSH Cucumis sativus 2-vinyl-4-ethyl chlorophyllide a + NADP+ i.e. monovinyl chlorophyllide a r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.75 30
-
-
 Cucumis sativus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.3.1.75 10 50 about half-maximal activity at 10°C and 50°C Cucumis sativus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.3.1.75 60
-
 complete inactivation after 2 min Cucumis sativus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.75 6.3
-
-
 Hordeum vulgare
1.3.1.75 6.3
-
-
 Zea mays
1.3.1.75 6.3
-
-
 Cucumis sativus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.1.75 5.5 8.5 about half-maximal activity at pH 5.5 and pH 8.5, no activity below pH 5.2 and above pH 11.0 Cucumis sativus