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Reactivity of glutaredoxins 1,2 and 3 from Escherichia coli shows that glutaredoxin 2 is the primary hydrogen donor to ArsC-catalazed arsenate reduction

Shi, J.; Vlamis-Gardikas, A.; Aslund, F.; Holmgren, A.; Rosen, B.P.; J. Biol. Chem. 274, 36039-36042 (1999)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.20.4.1
GSH
the enzyme uses GSH with glutaredoxin as electron donor
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.20.4.1
0.000003
-
glutaredoxin 2
-
Escherichia coli
1.20.4.1
0.0042
-
glutaredoxin 1
-
Escherichia coli
1.20.4.1
0.3
-
glutaredoxin 3
-
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.20.4.1
arsenate + reduced glutaredoxin
Escherichia coli
the enzyme is involved in bacterial arsenic resistance
arsenite + oxidized glutaredoxin
-
Escherichia coli
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.20.4.1
Escherichia coli
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.20.4.1
arsenate + reduced glutaredoxin
the enzyme uses GSH with glutaredoxin as electron donor. Glutaredoxin 2 is the most effective hydrogen donor for the reduction of arsenate. During the catalytic cycle, ArsC forms a mixed disulfide with GSH before being reduced by glutaredoxin to regenerate the active ArsC reductase
639367
Escherichia coli
arsenite + oxidized glutaredoxin
-
-
-
-
1.20.4.1
arsenate + reduced glutaredoxin
the enzyme is involved in bacterial arsenic resistance
639367
Escherichia coli
arsenite + oxidized glutaredoxin
-
639367
Escherichia coli
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.20.4.1
0.09
-
glutaredoxin 3
-
Escherichia coli
1.20.4.1
0.14
-
glutaredoxin 2
-
Escherichia coli
1.20.4.1
0.3
-
glutaredoxin 1
-
Escherichia coli
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.20.4.1
GSH
the enzyme uses GSH with glutaredoxin as electron donor
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.20.4.1
0.000003
-
glutaredoxin 2
-
Escherichia coli
1.20.4.1
0.0042
-
glutaredoxin 1
-
Escherichia coli
1.20.4.1
0.3
-
glutaredoxin 3
-
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.20.4.1
arsenate + reduced glutaredoxin
Escherichia coli
the enzyme is involved in bacterial arsenic resistance
arsenite + oxidized glutaredoxin
-
Escherichia coli
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.20.4.1
arsenate + reduced glutaredoxin
the enzyme uses GSH with glutaredoxin as electron donor. Glutaredoxin 2 is the most effective hydrogen donor for the reduction of arsenate. During the catalytic cycle, ArsC forms a mixed disulfide with GSH before being reduced by glutaredoxin to regenerate the active ArsC reductase
639367
Escherichia coli
arsenite + oxidized glutaredoxin
-
-
-
-
1.20.4.1
arsenate + reduced glutaredoxin
the enzyme is involved in bacterial arsenic resistance
639367
Escherichia coli
arsenite + oxidized glutaredoxin
-
639367
Escherichia coli
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.20.4.1
0.09
-
glutaredoxin 3
-
Escherichia coli
1.20.4.1
0.14
-
glutaredoxin 2
-
Escherichia coli
1.20.4.1
0.3
-
glutaredoxin 1
-
Escherichia coli