BRENDA - Enzyme Database show

Properties of the arsenate reductase of plasmid R773

Gladysheva, T.B.; Oden, K.L.; Rosen, B.P.; Biochemistry 33, 7288-7293 (1994)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.20.4.1
GSH
glutathione is not effective as electron donor
Escherichia coli
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.20.4.1
arsenite
-
Escherichia coli
1.20.4.1
NEM
-
Escherichia coli
1.20.4.1
phosphate
poor
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.20.4.1
8
-
arsenate
-
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.20.4.1
arsenate + reduced glutaredoxin
Escherichia coli
the enzyme is involved in bacterial arsenic resistance
arsenite + oxidized glutaredoxin
-
Escherichia coli
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.20.4.1
Escherichia coli
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.20.4.1
arsenate + reduced glutaredoxin
strong specificity for arsenate
639366
Escherichia coli
arsenite + oxidized glutaredoxin
-
-
-
-
1.20.4.1
arsenate + reduced glutaredoxin
thioredoxin is not effective as electron donor
639366
Escherichia coli
arsenite + oxidized glutaredoxin
-
-
-
-
1.20.4.1
arsenate + reduced glutaredoxin
the enzyme is involved in bacterial arsenic resistance
639366
Escherichia coli
arsenite + oxidized glutaredoxin
-
639366
Escherichia coli
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.20.4.1
additional information
-
reductase activity increases from 10C to 40C
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.20.4.1
6.3
6.8
-
Escherichia coli
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.20.4.1
GSH
glutathione is not effective as electron donor
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.20.4.1
arsenite
-
Escherichia coli
1.20.4.1
NEM
-
Escherichia coli
1.20.4.1
phosphate
poor
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.20.4.1
8
-
arsenate
-
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.20.4.1
arsenate + reduced glutaredoxin
Escherichia coli
the enzyme is involved in bacterial arsenic resistance
arsenite + oxidized glutaredoxin
-
Escherichia coli
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.20.4.1
arsenate + reduced glutaredoxin
strong specificity for arsenate
639366
Escherichia coli
arsenite + oxidized glutaredoxin
-
-
-
-
1.20.4.1
arsenate + reduced glutaredoxin
thioredoxin is not effective as electron donor
639366
Escherichia coli
arsenite + oxidized glutaredoxin
-
-
-
-
1.20.4.1
arsenate + reduced glutaredoxin
the enzyme is involved in bacterial arsenic resistance
639366
Escherichia coli
arsenite + oxidized glutaredoxin
-
639366
Escherichia coli
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.20.4.1
additional information
-
reductase activity increases from 10C to 40C
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.20.4.1
6.3
6.8
-
Escherichia coli