BRENDA - Enzyme Database show

Purification and characterization of Acr2p, the Saccharomyces cerevisiae arsenate reductase

Mukhopadhyay, R.; Shi, J.; Rosen, B.P.; J. Biol. Chem. 275, 21149-21157 (2000)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.20.4.1
GSH
required
Saccharomyces cerevisiae
Cloned(Commentary)
EC Number
Commentary
Organism
1.20.4.1
cloned into the bacterial expression vector and expressed in Escherichia coli as a C-terminal histidine-tagged protein
Saccharomyces cerevisiae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.20.4.1
arsenite
-
Saccharomyces cerevisiae
1.20.4.1
phosphate
poor
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.20.4.1
0.000002
-
glutaredoxin
glutaredoxin 2 from E. coli
Saccharomyces cerevisiae
1.20.4.1
0.0001
-
glutaredoxin
glutaredoxin 3 from E. coli
Saccharomyces cerevisiae
1.20.4.1
0.0006
-
glutaredoxin
glutaredoxin 1p from Saccharomyces cerevisiae or glutaredoxin 1 from E. coli
Saccharomyces cerevisiae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.20.4.1
16822
-
2 * 16822, calculation from nucleotide sequence
Saccharomyces cerevisiae
1.20.4.1
34000
-
gel filtration
Saccharomyces cerevisiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.20.4.1
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.20.4.1
-
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.20.4.1
arsenate + reduced glutaredoxin
thioredoxin is unable to support arsenate reduction. The N-terminal Cys residue is essential for arsenate reductase activity. During the catalytic cycle, Acr2p forms a mixed disulfide with GSH before being reduced vby glutaredoxin to regenerate the active Acr2p reductase
639363
Saccharomyces cerevisiae
arsenite + oxidized glutaredoxin
-
639363
Saccharomyces cerevisiae
?
Subunits
EC Number
Subunits
Commentary
Organism
1.20.4.1
dimer
2 * 16822, calculation from nucleotide sequence
Saccharomyces cerevisiae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.20.4.1
4.5
6.5
-
Saccharomyces cerevisiae
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.20.4.1
GSH
required
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.20.4.1
cloned into the bacterial expression vector and expressed in Escherichia coli as a C-terminal histidine-tagged protein
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.20.4.1
arsenite
-
Saccharomyces cerevisiae
1.20.4.1
phosphate
poor
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.20.4.1
0.000002
-
glutaredoxin
glutaredoxin 2 from E. coli
Saccharomyces cerevisiae
1.20.4.1
0.0001
-
glutaredoxin
glutaredoxin 3 from E. coli
Saccharomyces cerevisiae
1.20.4.1
0.0006
-
glutaredoxin
glutaredoxin 1p from Saccharomyces cerevisiae or glutaredoxin 1 from E. coli
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.20.4.1
16822
-
2 * 16822, calculation from nucleotide sequence
Saccharomyces cerevisiae
1.20.4.1
34000
-
gel filtration
Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.20.4.1
-
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.20.4.1
arsenate + reduced glutaredoxin
thioredoxin is unable to support arsenate reduction. The N-terminal Cys residue is essential for arsenate reductase activity. During the catalytic cycle, Acr2p forms a mixed disulfide with GSH before being reduced vby glutaredoxin to regenerate the active Acr2p reductase
639363
Saccharomyces cerevisiae
arsenite + oxidized glutaredoxin
-
639363
Saccharomyces cerevisiae
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.20.4.1
dimer
2 * 16822, calculation from nucleotide sequence
Saccharomyces cerevisiae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.20.4.1
4.5
6.5
-
Saccharomyces cerevisiae