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Literature summary extracted from

  • Muchmore, E.A.; Milewski, M.; Varki, A.; Diaz, S.
    Biosynthesis of N-glycolyneuraminic acid. The primary site of hydroxylation of N-acetylneuraminic acid is the cytosolic sugar nucleotide pool (1989), J. Biol. Chem., 264, 20216-20223.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.14.18.2 10 mM in the initial homogenization buffer is required for stability during 60-90 min incubation Mus musculus
1.14.18.2 10 mM in the initial homogenization buffer is required for stability during 60-90 min incubation Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.2 3'-AMP 5 mM, complete inhibition Sus scrofa
1.14.18.2 3'-CMP 5 mM, about 20% inhibition Sus scrofa
1.14.18.2 3'-UMP 5 mM, about 90% inhibition Sus scrofa
1.14.18.2 5'-AMP 5 mM, about 78% inhibition Sus scrofa
1.14.18.2 5'-UMP 5 mM, about 15% inhibition Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.2 0.6 0.9 CMP-N-acetylneuraminate pH 7.4, 37°C, enzyme from NS-1 cells Mus musculus
1.14.18.2 2.5
-
 CMP-N-acetylneuraminate pH 7.4, 37°C, enzyme from submaxillary gland Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.18.2 cytosol
-
 Mus musculus 5829
-
1.14.18.2 cytosol
-
 Sus scrofa 5829
-

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.2 Bos taurus
-
-
-
1.14.18.2 Mus musculus
-
-
-
1.14.18.2 Rattus norvegicus
-
-
-
1.14.18.2 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.18.2 colonic mucosa
-
 Rattus norvegicus
-
1.14.18.2 P3/NS1/1-Ag4.1 cell
-
 Mus musculus
-
1.14.18.2 P3X63Ag8 cell
-
 Mus musculus
-
1.14.18.2 submaxillary gland
-
 Sus scrofa
-
1.14.18.2 submaxillary gland
-
 Bos taurus
-

Storage Stability

EC Number Storage Stability Organism
1.14.18.2 -70°C, stable in crude extract for several weeks Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.2 additional information no activity towards free or alpha-glycosidically bound N-acetylneuraminic acid Mus musculus ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.18.2 6.4 7.4 enzyme preparation Mus musculus
1.14.18.2 6.4 7.4 enzyme preparation Sus scrofa