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Literature summary extracted from

  • Zhang, Z.; Schofield, C.J.; Baldwin, J.E.; Thomas, P.; John, P.
    Expression, purification and characterization of 1-aminocyclopropane-1-carboxylate oxidase from tomato in Escherichia coli (1995), Biochem. J., 307, 77-85.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.17.4 ascorbate required Solanum lycopersicum
1.14.17.4 ascorbate cannot be replaced by DTT or NADH Solanum lycopersicum
1.14.17.4 bovine serum albumin stimulates the recombinant enzyme by 41% at 0.1 mM Solanum lycopersicum
1.14.17.4 catalase recombinant enzyme, stimulates by 36% at 0.5 mg/ml Solanum lycopersicum
1.14.17.4 CO2 requires HCO3-/CO2 mixture Solanum lycopersicum
1.14.17.4 dithiothreitol recombinant enzyme, stimulates by 38% at 1 mM Solanum lycopersicum
1.14.17.4 ethylene stimulates Solanum lycopersicum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.17.4 overexpression in Escherichia coli BL21(DE3) Solanum lycopersicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.17.4 diethyl dicarbonate complete inactivation at 1.5 mM, pH 6.8, after 20 min Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.17.4 0.0053
-
 Fe2+ recombinant enzyme, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C Solanum lycopersicum
1.14.17.4 0.032
-
 1-aminocyclopropane-1-carboxylate recombinant enzyme, in presence of 5mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C Solanum lycopersicum
1.14.17.4 1
-
 5,6-O-isopropylidine L-ascorbate recombinant enzyme, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C Solanum lycopersicum
1.14.17.4 1.7
-
 D-ascorbate recombinant enzyme, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C Solanum lycopersicum
1.14.17.4 16.7
-
 L-ascorbate recombinant enzyme, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C Solanum lycopersicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.17.4 35820
-
 electron spray mass spectrometry Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.17.4 1-aminocyclopropane-1-carboxylate + ascorbate + O2 Solanum lycopersicum
-
 ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.17.4 Solanum lycopersicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.17.4 recombinant from E. coli, 9.8fold to near homogeneity Solanum lycopersicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.17.4 0.035
-
 purified enzyme Solanum lycopersicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.17.4 1-amino-2-ethylcyclopropane-1-carboxylate + L-ascorbate + O2 prefers mixture of 1R,2S- and 1S,2R-amino-2-ethylcyclopropane-1-carboxylate, less effective with racemic mixture of 1R,2R- and 1S,2S-diastereomers Solanum lycopersicum 1-butene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?
1.14.17.4 1-aminocyclopropane-1-carboxylate + 5,6-O-isopropylidine L-ascorbate + O2 better cosubstrate than L-ascorbate Solanum lycopersicum ethylene + cyanide + 5,6-O-isopropylidine L-dehydroascorbate + CO2 + H2O
-
 ?
1.14.17.4 1-aminocyclopropane-1-carboxylate + ascorbate + O2
-
 Solanum lycopersicum ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?
1.14.17.4 1-aminocyclopropane-1-carboxylate + D-ascorbate + O2 better cosubstrate than L-ascorbate Solanum lycopersicum ethylene + cyanide + D-dehydroascorbate + CO2 + H2O
-
 ?
1.14.17.4 1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 i.e. ACC Solanum lycopersicum ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?
1.14.17.4 additional information L-galactono-gamma-lactone is no cosubstrate Solanum lycopersicum ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.17.4 28
-
 assay at Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.17.4 7.2
-
 assay at Solanum lycopersicum