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Literature summary extracted from

  • Bouwmeester, H.J.; Konings, M.C.J.M.; Gershenzon, J.; Karp, F.; Croteau, R.
    Cytochrome P-450 dependent (+)-limonene-6-hydroxylation in fruits of caraway (Carum Carvi) (1999), Phytochemistry, 50, 243-248.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.14.14.53 polyvinylpyrrolidone, NaHsO3, sodium diethyldithiocarbamate, leupeptin, EGTA, catalase, and glutathione stabilize microsomal extract Carum carvi

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.53 clotrimazole less than 0.001 mM, 50% inhibition Carum carvi
1.14.14.53 cytochrome c less than 0.01 mM, 50% inhibition Carum carvi
1.14.14.53 metapyrone 1.5 mM, 50% inhibition Carum carvi
1.14.14.53 miconazole 0.048 mM, 50% inhibition Carum carvi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.53 0.0114
-
(+)-limonene annual caraway Carum carvi
1.14.14.53 0.0149
-
(+)-limonene biannual caraway Carum carvi

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.53 microsome
-
Carum carvi
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.53 Carum carvi
-
var Karzo, annual caraway, var Beija, biennial caraway
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.53 fruit
-
Carum carvi
-

Storage Stability

EC Number Storage Stability Organism
1.14.14.53 -80 C, microsomal preparation, 1 year, no loss of activity Carum carvi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.53 (+)-limonene + NADPH + H+ + O2 high regio- and stereospecificity, requiring NADPH Carum carvi (+)-trans-carveol + NADP+ + H2O 97% of total product, 2.5% (-)-trans-carveol, 0.5% (-)-cis-carveol ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.53 7.2 7.4
-
Carum carvi

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.14.53 6.3 8.4
-
Carum carvi

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.53 cytochrome P-450
-
Carum carvi
1.14.14.53 NADP+ no activity with NAD+ Carum carvi