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Literature summary extracted from

  • Kuehn, H.; Barnett, J.; Grunberger, D.; Baecker, P.; Chow, J.; Nguyen, B.; Bursztyn-Pettegrew, H.; Chan, H.; Sigal, E.
    Overexpression, purification and characterization of human recombinant 15-lipoxygenase (1993), Biochim. Biophys. Acta, 1169, 80-89.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.33 expression to high levels, approximately 20% of cellular protein, in a baculovirus/insect cell expression system Homo sapiens

General Stability

EC Number General Stability Organism
1.13.11.33 repeated freeze thawing especially in the absence of gylcerol results in 60% reduction of specific activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.33 5,8,11,14-Eicosatetraynoic acid inhibits reaction of linoleic acid Homo sapiens
1.13.11.33 additional information the enzyme undergoes suicidal inactivation, during fatty acid oxygenation Homo sapiens
1.13.11.33 nordihydroguaiaretic acid
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.33 0.003
-
 linoleic acid
-
 Homo sapiens
1.13.11.33 0.012
-
 arachidonic acid
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.13.11.33 cytosol
-
 Homo sapiens 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.33 Fe2+ addition in a molar excess, more than 50:1 purified protein:Fe2+, leads to a 3fold increase in the specific activity Homo sapiens
1.13.11.33 Iron the recombinant enzyme contains a variable amount of iron, often as low as 0.2 mol per mol of enzyme Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.33 72000
-
 x * 72000, SDS-PAGE Homo sapiens
1.13.11.33 74727
-
 x * 74727, electrospray mass spectrometry Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.33 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.13.11.33 glycoprotein contains 5% carbohydrate Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.33
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
1.13.11.33 -70°C, 5% glycerol, stable for more than 6 months Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.33 arachidonate + O2
-
 Homo sapiens (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyeicosa-5,8,11,13-tetraenoate formation of 15S-hydroxyeicosatetraenoic acid and 12S-hydroxyeicosatetraenoic acid in a ratio of 12:1, double oxygenation products 14R,15S-dihydroxyeicosatetraenoic acid and various 8,15-dihydroxyeicosatetraenoic acids are also produced ?
1.13.11.33 linoleic acid + O2
-
 Homo sapiens 13-hydroxylinoleic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.33 ? x * 72000, SDS-PAGE Homo sapiens
1.13.11.33 ? x * 74727, electrospray mass spectrometry Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.33 7
-
-
 Homo sapiens