EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.86 | 2-dimethylphosphinoyl-2-hydroxyacetic acid | competitive, reversible | Hordeum vulgare |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.86 | 0.0043 | - |
NADPH | pH 7.5, 25°C | Hordeum vulgare | |
1.1.1.86 | 0.011 | - |
2-acetolactate | pH 7.5, 25°C | Hordeum vulgare | |
1.1.1.86 | 0.038 | - |
2-aceto-2-hydroxybutyrate | pH 7.5, 25°C | Hordeum vulgare |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.86 | Mg2+ | required | Hordeum vulgare |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.86 | 59000 | - |
2 * 59000, SDS-PAGE | Hordeum vulgare |
1.1.1.86 | 115000 | - |
gel filtration | Hordeum vulgare |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.86 | 2-acetolactate + NADPH | Hordeum vulgare | enzyme of branched chain amino acid synthesis | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.86 | Hordeum vulgare | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.86 | - |
Hordeum vulgare |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.86 | shoot | - |
Hordeum vulgare | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.86 | 3.04 | - |
- |
Hordeum vulgare |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.86 | 2-aceto-2-hydroxybutyrate + NADPH + H+ | - |
Hordeum vulgare | 2,3-dihydroxy-3-methylvalerate + NADP+ | - |
r | |
1.1.1.86 | 2-acetolactate + NADPH | enzyme of branched chain amino acid synthesis | Hordeum vulgare | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r | |
1.1.1.86 | NADPH + 2-acetolactate | - |
Hordeum vulgare | NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.86 | dimer | 2 * 59000, SDS-PAGE | Hordeum vulgare |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.86 | 7.5 | - |
2-acetolactate as substrate | Hordeum vulgare |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.86 | NADPH | - |
Hordeum vulgare |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.86 | 0.00019 | - |
2-dimethylphosphinoyl-2-hydroxyacetic acid | pH 7.5, 25°C, with 2-aceto-2-hydroxybutyrate as substrate | Hordeum vulgare | |
1.1.1.86 | 0.00046 | - |
2-dimethylphosphinoyl-2-hydroxyacetic acid | pH 7.5, 25°C, with 2-acetolactate as substrate | Hordeum vulgare |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.1.1.86 | Hordeum vulgare | chromatofocusing | 4.7 | 4.6 |