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Literature summary extracted from

  • Satyanarayana, T.; Radhakrishnan, A.N.
    Biosynthesis of valine and isoleucine in plants. 3. Reductoisomerase of Phaseolus radiatus (1965), Biochim. Biophys. Acta, 110, 380-388.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.86 ascorbic acid 5 mM, enhances activity 2-hydroxy-2-ethyl-3-oxobutanoate as substrate Vigna radiata var. radiata

General Stability

EC Number General Stability Organism
1.1.1.86 high activity loss during DEAE-cellulose chromatography, stable towards acetone fractionation and dialysis, unstable towards freezing and thawing Vigna radiata var. radiata

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.86 arsenite slight inhibition Vigna radiata var. radiata
1.1.1.86 ascorbic acid slight inhibition with 2-acetolactate as substrate Vigna radiata var. radiata
1.1.1.86 L-ascorbic acid slightly inhibitory with alpha-acetolactate as substrate Vigna radiata var. radiata
1.1.1.86 additional information no inhibition with p-mercuribenzoate, cysteine, 2-mercaptoethanol Vigna radiata var. radiata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.86 0.12
-
 2-aceto-2-hydroxybutyrate
-
 Vigna radiata var. radiata
1.1.1.86 5.5
-
 2-acetolactate
-
 Vigna radiata var. radiata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.86 additional information no metal ions required Vigna radiata var. radiata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.86 2-acetolactate + NADPH Vigna radiata var. radiata enzyme of branched chain amino acid synthesis 2,3-dihydroxy-3-methylbutanoate + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.86 Vigna radiata var. radiata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.86 partial Vigna radiata var. radiata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.86 2-acetolactate + NADPH enzyme of branched chain amino acid synthesis Vigna radiata var. radiata 2,3-dihydroxy-3-methylbutanoate + NADP+
-
 r
1.1.1.86 NADPH + 2-aceto-2-hydroxybutyrate
-
 Vigna radiata var. radiata NADP+ + ?
-
 ?
1.1.1.86 NADPH + 2-acetolactate
-
 Vigna radiata var. radiata NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.86 6.5 9 constant activity with 2-acetolactate as substrate Vigna radiata var. radiata
1.1.1.86 8.6
-
 with 2-hydroxy-2-ethyl-3-oxobutanoate as substrate, Tris-HCl buffer, alpha-aceto-alpha-hydroxybutyrate as substrate, Tris buffer Vigna radiata var. radiata

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.86 7 9 pH 7.0: about 40% of maximal activity, pH 9.0: about 90% of maximal activity, with 2-hydroxy-2-ethyl-3-oxobutanoate as substrate, Tris-HCl buffer Vigna radiata var. radiata

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.86 NADPH
-
 Vigna radiata var. radiata