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Literature summary extracted from

  • Kawaguchi, H.; Inagaki, K.; Kuwata, Y.; Tanaka, H.; Tano, T.
    3-Isopropylmalate dehydrogenase from chemolithoautotroph Thiobacillus ferrooxidans: DNA sequence, enzyme purification, and characterization (1993), J. Biochem., 114, 370-377.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.85 iodoacetamide 5 mM, 50% inhibition Acidithiobacillus ferrooxidans
1.1.1.85 iodoacetate 5 mM, 50% inhibition Acidithiobacillus ferrooxidans
1.1.1.85 NEM 5 mM, 50% inhibition Acidithiobacillus ferrooxidans
1.1.1.85 PCMB 5 mM, 50% inhibition Acidithiobacillus ferrooxidans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.85 0.026
-
 (2R,3S)-3-isopropylmalate pH 9.0 Acidithiobacillus ferrooxidans
1.1.1.85 0.8
-
 NAD+ pH 9.0 Acidithiobacillus ferrooxidans
1.1.1.85 4
-
 D-malate pH 9.0 Acidithiobacillus ferrooxidans
1.1.1.85 12
-
 L-malate pH 9.0 Acidithiobacillus ferrooxidans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.85 K+ monovalent cation required Acidithiobacillus ferrooxidans
1.1.1.85 Mg2+ 1.0 mM, enhances activity 4.7fold Acidithiobacillus ferrooxidans
1.1.1.85 Mn2+ 1.0 mM, enhances activity 4.2fold Acidithiobacillus ferrooxidans
1.1.1.85 NH4+ monovalent cation required Acidithiobacillus ferrooxidans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.85 38000
-
 2 * 38000, SDS-PAGE Acidithiobacillus ferrooxidans
1.1.1.85 58000
-
 gel filtration Acidithiobacillus ferrooxidans
1.1.1.85 73000
-
 equilibrium sedimentation Acidithiobacillus ferrooxidans

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.85 Acidithiobacillus ferrooxidans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.85 recombinant enzyme expressed in Escherichia coli Acidithiobacillus ferrooxidans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.85 138.2
-
-
 Acidithiobacillus ferrooxidans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+
-
 Acidithiobacillus ferrooxidans 2-oxoisocaproate + NADH + H+ + CO2
-
 ?
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+
-
 Acidithiobacillus ferrooxidans (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
 r
1.1.1.85 (S)-malate + NAD+ 12% of the activity with 3-isopropylmalate Acidithiobacillus ferrooxidans pyruvate + NADH + CO2
-
 ?
1.1.1.85 D-malate + NAD+ 19% of the activity with 3-isopropylmalate Acidithiobacillus ferrooxidans pyruvate + NADH + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.85 dimer 2 * 38000, SDS-PAGE Acidithiobacillus ferrooxidans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.85 60
-
-
 Acidithiobacillus ferrooxidans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.85 70
-
 10 min, 40% loss of activity Acidithiobacillus ferrooxidans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.85 9
-
-
 Acidithiobacillus ferrooxidans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.85 NAD+
-
 Acidithiobacillus ferrooxidans