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Literature summary extracted from

  • Parsons, S.J.; Burns, R.O.
    Purification and properties of beta-isopropylmalate dehydrogenase (1969), J. Biol. Chem., 244, 996-1003.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.85 iodoacetamide 50 mM, slight inhibition Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.85 iodoacetate 50 mM, slight inhibition Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.85 K+ above 1 M, slight inhibition Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.85 N-ethylmaleimide
-
 Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.85 NEM
-
 Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.85 p-hydroxymercuribenzoate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.85 PCMB
-
 Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.85 0.019
-
 (2R,3S)-3-isopropylmalate pH 9.0 Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.85 0.1
-
 NAD+ pH 9.0 Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.85 35000
-
 2 * 35000, sedimentation equilibrium centrifugation after treatment with mercaptoethanol and 6 M guanidine-HCl Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.85 70000
-
 equilibrium sedimentation Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+ Salmonella enterica subsp. enterica serovar Typhimurium enzyme is involved in leucine biosynthesis 2-oxoisocaproate + NADH + H+ + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.85 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.85
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+
-
 Salmonella enterica subsp. enterica serovar Typhimurium 2-oxoisocaproate + NADH + H+ + CO2
-
 ?
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+ enzyme is involved in leucine biosynthesis Salmonella enterica subsp. enterica serovar Typhimurium 2-oxoisocaproate + NADH + H+ + CO2
-
 ?
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+
-
 Salmonella enterica subsp. enterica serovar Typhimurium (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
 r
1.1.1.85 (2R,3S)-3-isopropylmalate + thionicotinamide NAD+
-
 Salmonella enterica subsp. enterica serovar Typhimurium 2-oxoisohexanoate + thionicotinamideNADH + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.85 dimer 2 * 35000, sedimentation equilibrium centrifugation after treatment with mercaptoethanol and 6 M guanidine-HCl Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.85 9 9.5
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.85 NAD+
-
 Salmonella enterica subsp. enterica serovar Typhimurium