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Literature summary extracted from

  • Sekiguchi, T.; Suda, M.; Sekiguchi, T.; Nosoh, Y.
    Cloning and DNA homology of 3-isopropylmalate dehydrogenase genes from thermophilic bacilli (1986), FEMS Microbiol. Lett., 36, 41-45.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.85 expression in Escherichia coli using pBR322 as a vector [Bacillus] caldotenax
1.1.1.85 expression in Escherichia coli using pBR322 as a vector [Bacillus] caldolyticus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.85 Bacillus subtilis
-
-
-
1.1.1.85 Escherichia coli
-
-
-
1.1.1.85 Saccharomyces cerevisiae
-
-
-
1.1.1.85 Thermus thermophilus
-
-
-
1.1.1.85 [Bacillus] caldolyticus
-
-
-
1.1.1.85 [Bacillus] caldotenax
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+
-
 Bacillus subtilis 2-oxoisocaproate + NADH + H+ + CO2
-
 ?
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+
-
 Thermus thermophilus 2-oxoisocaproate + NADH + H+ + CO2
-
 ?
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+
-
 Escherichia coli 2-oxoisocaproate + NADH + H+ + CO2
-
 ?
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+
-
 Saccharomyces cerevisiae 2-oxoisocaproate + NADH + H+ + CO2
-
 ?
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+
-
 [Bacillus] caldotenax 2-oxoisocaproate + NADH + H+ + CO2
-
 ?
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+
-
 [Bacillus] caldolyticus 2-oxoisocaproate + NADH + H+ + CO2
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.85 NAD+
-
 Bacillus subtilis
1.1.1.85 NAD+
-
 Thermus thermophilus
1.1.1.85 NAD+
-
 Escherichia coli
1.1.1.85 NAD+
-
 Saccharomyces cerevisiae
1.1.1.85 NAD+
-
 [Bacillus] caldotenax
1.1.1.85 NAD+
-
 [Bacillus] caldolyticus