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Literature summary extracted from

  • Onodera, K.; Moriyama, H.; Takenaka, A.; Tanaka, N.; Akutsu, N.; Muro, M.; Oshima, T.; Imada, K.; Sato, M.; Katsube, Y.
    Crystallization and preliminary X-ray studies of a Bacillus subtilis and Thermus thermophilus HB8 chimeric 3-isopropylmalate dehydrogenase (1991), J. Biochem., 109, 1-2.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.85 construction of a chimeric gene by fusing the Bacillus subtilis and Thermus thermophilus genes coding for 3-isopropylmalate dehydrogenase, expression in Escherichia coli Bacillus subtilis
1.1.1.85 construction of a chimeric gene by fusing the Bacillus subtilis and Thermus thermophilus genes coding for 3-isopropylmalate dehydrogenase, expression in Escherichia coli Thermus thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.85 hanging drop vapor diffusion method, chimeric enzyme constructed by fusing the gene of Bacillus subtilis and Thermus thermophilus coding for 3-isopropylmalate dehydrogenase, expression in Escherichia coli Bacillus subtilis
1.1.1.85 hanging drop vapor diffusion method, chimeric enzyme constructed by fusing the gene of Bacillus subtilis and Thermus thermophilus coding for 3-isopropylmalate dehydrogenase, expression in Escherichia coli Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.85 Bacillus subtilis
-
-
-
1.1.1.85 Thermus thermophilus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+
-
 Bacillus subtilis 2-oxoisocaproate + NADH + H+ + CO2
-
 ?
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+
-
 Thermus thermophilus 2-oxoisocaproate + NADH + H+ + CO2
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.85 NAD+
-
 Bacillus subtilis
1.1.1.85 NAD+
-
 Thermus thermophilus