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Literature summary extracted from

  • Yokota, A.; Sasajima, K.; Yoneda, M.
    Reactivation of inactivated D-glucose dehydrogenase of a Bacillus species by pyridine and adenine nucleotides (1979), Agric. Biol. Chem., 43, 271-278.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.47 ADP NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion Bacillus sp. (in: Bacteria)
1.1.1.47 AMP NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion Bacillus sp. (in: Bacteria)
1.1.1.47 ATP NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion Bacillus sp. (in: Bacteria)
1.1.1.47 dipicolinic acid NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion Bacillus sp. (in: Bacteria)
1.1.1.47 NAD+ NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion Bacillus sp. (in: Bacteria)
1.1.1.47 NADH NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion Bacillus sp. (in: Bacteria)
1.1.1.47 NADP+ NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion Bacillus sp. (in: Bacteria)
1.1.1.47 NADPH NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion Bacillus sp. (in: Bacteria)

General Stability

EC Number General Stability Organism
1.1.1.47 crude enzyme loses 90% activity after 3 days at 5°C Bacillus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.47 0.061
-
 NADP+
-
 Bacillus sp. (in: Bacteria)
1.1.1.47 0.082
-
 NAD+
-
 Bacillus sp. (in: Bacteria)
1.1.1.47 4.2
-
 beta-D-glucose
-
 Bacillus sp. (in: Bacteria)

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.47 (NH4)2SO4 NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion Bacillus sp. (in: Bacteria)
1.1.1.47 KCl NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion Bacillus sp. (in: Bacteria)
1.1.1.47 Na2SO4 NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion Bacillus sp. (in: Bacteria)
1.1.1.47 NaCl NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.47 45000
-
 inactive enzyme form, gel filtration Bacillus sp. (in: Bacteria)
1.1.1.47 80000
-
 active enzyme form, gel filtration Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.47 Bacillus sp. (in: Bacteria)
-
 D-ribose producing mutant
-
1.1.1.47 Bacillus sp. (in: Bacteria) BG 1722
-
 D-ribose producing mutant
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.47 DEAE-cellulose, partially purified enzyme, 10 mM NAD(P)+ protects from inactivation on column Bacillus sp. (in: Bacteria)

Storage Stability

EC Number Storage Stability Organism
1.1.1.47 4°C, 89% loss of activity after 4 days without stabilzing agent, complete stabilization by 10 mM NAD+ or 10 mM NADP+, and to a lesser extent by 800 mM NaCl. Partial stabilization by 800 MM (NH4)2SO4, 800 mM Na2SO4 or 800 mM dipicolinic acid Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.47 2-deoxy-D-glucose + NAD+ 113% of the activity with D-glucose Bacillus sp. (in: Bacteria) 2-deoxy-D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.47 2-deoxy-D-glucose + NAD+ 113% of the activity with D-glucose Bacillus sp. (in: Bacteria) BG 1722 2-deoxy-D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.47 2-deoxy-D-glucose + NADP+ 103% of the activity with D-glucose and NAD+ Bacillus sp. (in: Bacteria) 2-deoxy-D-glucono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.47 2-deoxy-D-glucose + NADP+ 103% of the activity with D-glucose and NAD+ Bacillus sp. (in: Bacteria) BG 1722 2-deoxy-D-glucono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.47 beta-D-glucose + NADP+ 93% of the activity with NAD+ Bacillus sp. (in: Bacteria) D-glucono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.47 beta-D-glucose + NADP+ 93% of the activity with NAD+ Bacillus sp. (in: Bacteria) BG 1722 D-glucono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.47 maltose + NAD+ 6% of the activity with D-glucose Bacillus sp. (in: Bacteria) ? + NADH
-
 ?
1.1.1.47 maltose + NAD+ 6% of the activity with D-glucose Bacillus sp. (in: Bacteria) BG 1722 ? + NADH
-
 ?
1.1.1.47 maltose + NADP+ 5% of the activity D-glucose and NAD+ Bacillus sp. (in: Bacteria) ? + NADPH
-
 ?
1.1.1.47 maltose + NADP+ 5% of the activity D-glucose and NAD+ Bacillus sp. (in: Bacteria) BG 1722 ? + NADPH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.47 More
-
 Bacillus sp. (in: Bacteria)
1.1.1.47 tetramer 2 * 45000, gel filtration of inactive monomeric enzyme Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.47 50
-
-
 Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.47 9
-
-
 Bacillus sp. (in: Bacteria)

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.47 NAD+
-
 Bacillus sp. (in: Bacteria)
1.1.1.47 NADP+
-
 Bacillus sp. (in: Bacteria)