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Literature summary extracted from

  • Carper, W.R.; Campbell, D.P.; Morrical, S.W.; Thompson, R.E.
    Characterization studies of glucose dehydrogenase (1983), Experientia, 39, 1295-1297.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.47 0.0024
-
 beta-D-glucose pH 7.5, 37°C, coenzyme NAD+, 37°C Sus scrofa
1.1.1.47 0.0029
-
 D-Glucose-6-phosphate 37°C, pH 7.5, coenzyme NADP+ Sus scrofa
1.1.1.47 0.0032
-
 beta-D-glucose pH 10.0, 37°C, coenzyme NAD+ Sus scrofa
1.1.1.47 0.0036
-
 NADP+ pH 7.5, 37°C, reaction with D-glucose-6-phosphate Sus scrofa
1.1.1.47 0.0046
-
 NADP+ pH 10.0, 37°C, reaction with beta-D-glucose Sus scrofa
1.1.1.47 0.0051
-
 NAD+ pH 7.5, 37°C, reaction with beta-D-glucose Sus scrofa
1.1.1.47 0.0072
-
 D-Glucose-6-phosphate pH 10.0, 37°C, coenzyme NADP+ Sus scrofa
1.1.1.47 0.011
-
 beta-D-glucose pH 7.5, 37°C, coenzyme NADP+ Sus scrofa
1.1.1.47 0.013
-
 NADP+ pH 7.5, 37°C, reaction with beta-D-glucose Sus scrofa
1.1.1.47 0.028
-
 NADP+ pH 10.0, 37°C, reaction with beta-D-glucose Sus scrofa
1.1.1.47 0.032
-
 beta-D-glucose pH 10.0, 37°C, coenzyme NADP+ Sus scrofa
1.1.1.47 0.062
-
 NADP+ pH 10.0, 37°C, reaction with D-glucose-6-phosphate Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.47 endoplasmic reticulum
-
 Sus scrofa 5783
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.47 58000
-
 4 * 58000, SDS-PAGE Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.47 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.47 Triton X-114, acid treatment, ammonium sulfate, Sephacryl S-200, Sephacryl S-300, SE-cellulose, DEAE-cellulose, purified enzyme contains 1.7% lipid Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.47 liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.47 3.57
-
-
 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.47 beta-D-glucose + NAD+
-
 Sus scrofa D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.47 beta-D-glucose + NADP+
-
 Sus scrofa D-glucono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.47 beta-D-glucose 6-phosphate + NADP+
-
 Sus scrofa D-glucono-1,5-lactone 6-phosphate + NADPH
-
 ?
1.1.1.47 D-glucose 6-phosphate + NADP+
-
 Sus scrofa D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.47 tetramer 4 * 58000, SDS-PAGE Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.47 10
-
 at 37°C, 50 mM glycine buffer Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.47 NADP+
-
 Sus scrofa