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Literature summary extracted from

  • Juhasz, A.; Csizmadia, V.; Borbely, G.; Udvardy, J.; Farkas, G.L.
    The pyridine nucleotide-dependent D-glucose dehydrogenase of Nostoc sp. strain Mac, a cyanobacterium, is subject to thioredoxin modulation (1986), FEBS Lett., 194, 121-125.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.47 thioredoxin in the presence of 0.5 mM dithiothreitol Nostoc sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.47 0.024
-
NADP+ pH 7.5 Nostoc sp.
1.1.1.47 0.285
-
NADP+ pH 7.5, enzyme reduced with Nostoc sp. thioredoxin Nostoc sp.
1.1.1.47 14
-
beta-D-glucose pH 7.5 Nostoc sp.
1.1.1.47 15
-
beta-D-glucose pH 7.5, enzyme reduced with Nostoc sp. thioredoxin Nostoc sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.47 Nostoc sp.
-
-
-
1.1.1.47 Nostoc sp. MAC
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.47 ammonium sulfate, hydroxyapatite, Sephacryl S-200 Nostoc sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.47 105
-
-
Nostoc sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.47 beta-D-glucose + NAD+ about 3times more active with NADP+ than with NAD+ Nostoc sp. D-glucono-1,5-lactone + NADH + H+
-
?
1.1.1.47 beta-D-glucose + NAD+ about 3times more active with NADP+ than with NAD+ Nostoc sp. MAC D-glucono-1,5-lactone + NADH + H+
-
?
1.1.1.47 beta-D-glucose + NADP+ about 3times more active with NADP+ than with NAD+ Nostoc sp. D-glucono-1,5-lactone + NADPH + H+
-
?
1.1.1.47 beta-D-glucose + NADP+ about 3times more active with NADP+ than with NAD+ Nostoc sp. MAC D-glucono-1,5-lactone + NADPH + H+
-
?

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.47 NAD+
-
Nostoc sp.
1.1.1.47 NADP+ about 3times more active with NADP+ than with NAD+ Nostoc sp.