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Literature summary extracted from

  • Giardina, P.; DeBasia, M.G.; DeRosa, M.; Gambacort, A.; Buonocore, V.
    Glucose dehydrogenase from the thermoacidophilic archaebacterium Sulfolobus solfataricus (1986), Biochem. J., 239, 517-522.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.1.1.47 0.05% SDS, no loss of activity, 0.1%, slow decrease Saccharolobus solfataricus
1.1.1.47 2 M urea, 24 h, room temperature, no loss of activity, slow decrease at 4 M Saccharolobus solfataricus
1.1.1.47 4 M guanidinium chloride, rapid inactivation Saccharolobus solfataricus
1.1.1.47 H4-furan, rapid inactivation Saccharolobus solfataricus
1.1.1.359 dehydrogenase preparations became inactivated irreversibly when stored in the absence of ethylene glycol and Mg2+, at very low protein concentration, or during freezing and thawing Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.47 2-mercaptoethanol 1 mM, room temperature, pH 9.0, rapid inactivation Saccharolobus solfataricus
1.1.1.47 5,5'-dithiobis(2-nitrobenzoic acid) 1 mM, room temperature, pH 9.0, 50% inactivation Saccharolobus solfataricus
1.1.1.47 EDTA 50 mM, 5% activity left Saccharolobus solfataricus
1.1.1.47 N-ethylmaleimide 3 mM, room temperature, pH 9.0, 50% inactivation Saccharolobus solfataricus
1.1.1.118 2-mercaptoethanol rapid inactivation at 1 mM Saccharolobus solfataricus
1.1.1.118 5,5'-dithiobis-(2-nitrobenzoic acid)
-
 Saccharolobus solfataricus
1.1.1.118 EDTA 5% residual activity at 50 mM, addition of Mg2+ in excess restored the initial activity Saccharolobus solfataricus
1.1.1.118 Guanidinium chloride rapid inactivation at 4 M Saccharolobus solfataricus
1.1.1.118 additional information D-alactose, D-mannose and D-ribose, in concentrations as high as 0.04 mM do not inhibit glucose oxidation by NAD+ Saccharolobus solfataricus
1.1.1.118 N-ethylmaleimide
-
 Saccharolobus solfataricus
1.1.1.118 NADPH inhibits the NAD+-dependent carbohydrate oxidations in a competitive manner with respect to NAD+ Saccharolobus solfataricus
1.1.1.118 tetrahydrofuran
-
 Saccharolobus solfataricus
1.1.1.359 2-mercaptoethanol 1 mM, pH 9, 22°C, rapid inactivation Saccharolobus solfataricus
1.1.1.359 5,5'-dithiobis-(2-nitrobenzoic acid) 1 mM, 50% inactivation Saccharolobus solfataricus
1.1.1.359 EDTA 50 mM, 95% inhibition Saccharolobus solfataricus
1.1.1.359 additional information galactose (0.04 mM), mannose (0.04 mM) and ribose (0.04 mM) do not inhibit glucose oxidation by NAD+ Saccharolobus solfataricus
1.1.1.359 NADPH inhibits the NAD+-dependentcarbohydrate oxidations in a competitive manner with respect to NAD+ Saccharolobus solfataricus
1.1.1.359 NEM 3 mM, 2 h, 50% inactivation Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.47 0.03
-
 NADP+ pH 9.0, 70°C Saccharolobus solfataricus
1.1.1.47 0.44
-
 beta-D-glucose pH 9.0, 70°C, coenzyme NADP+ Saccharolobus solfataricus
1.1.1.47 1.2
-
 NAD+ pH 9.0, 70°C Saccharolobus solfataricus
1.1.1.47 2.2
-
 beta-D-glucose pH 9.0, 70°C, coenzyme NADP+ Saccharolobus solfataricus
1.1.1.47 8
-
 beta-D-glucose pH 9.0, 70°C, coenzyme NAD+ Saccharolobus solfataricus
1.1.1.47 22
-
 beta-D-galactose pH 9.0, 70°C, coenzyme NADP+ Saccharolobus solfataricus
1.1.1.47 68
-
 beta-D-xylose pH 9.0, 70°C, coenzyme NAD+ Saccharolobus solfataricus
1.1.1.118 0.03
-
 NADP+ in the presence of D-galactose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C Saccharolobus solfataricus
1.1.1.118 0.03
-
 NADP+ in the presence of D-glucose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C Saccharolobus solfataricus
1.1.1.118 0.03
-
 NADP+ in the presence of D-xylose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C Saccharolobus solfataricus
1.1.1.118 0.44
-
 D-glucose in the presence of NADP+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C Saccharolobus solfataricus
1.1.1.118 1.2
-
 NAD+ in the presence of D-glucose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C Saccharolobus solfataricus
1.1.1.118 1.2
-
 NAD+ in the presence of D-xylose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C Saccharolobus solfataricus
1.1.1.118 2.2
-
 D-xylose in the presence of NADP+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C Saccharolobus solfataricus
1.1.1.118 8
-
 D-glucose in the presence of NAD+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C Saccharolobus solfataricus
1.1.1.118 22
-
 D-galactose in the presence of NADP+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C Saccharolobus solfataricus
1.1.1.118 68
-
 D-xylose in the presence of NAD+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70°C Saccharolobus solfataricus
1.1.1.359 0.03
-
 NADP+ pH 9.0, 70°C, cosubstrate: beta-D-glucose Saccharolobus solfataricus
1.1.1.359 0.03
-
 NADP+ pH 9.0, 70°C, cosubstrate: D-galactose Saccharolobus solfataricus
1.1.1.359 0.03
-
 NADP+ pH 9.0, 70°C, cosubstrate: D-xylose Saccharolobus solfataricus
1.1.1.359 0.44
-
 beta-D-glucose pH 9.0, 70°C, cosubstrate: NADP+ Saccharolobus solfataricus
1.1.1.359 1.2
-
 NAD+ pH 9.0, 70°C, cosubstrate: beta-D-glucose Saccharolobus solfataricus
1.1.1.359 1.2
-
 NAD+ pH 9.0, 70°C, cosubstrate: D-xylose Saccharolobus solfataricus
1.1.1.359 2.2
-
 D-xylose pH 9.0, 70°C, cosubstrate: NADP+ Saccharolobus solfataricus
1.1.1.359 8
-
 beta-D-glucose pH 9.0, 70°C, cosubstrate: NAD+ Saccharolobus solfataricus
1.1.1.359 22
-
 D-galactose pH 9.0, 70°C, cosubstrate: NADP+ Saccharolobus solfataricus
1.1.1.359 68
-
 D-xylose pH 9.0, 70°C, cosubstrate: NAD+ Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.47 Ca2+ maximal activity at 20 mM Saccharolobus solfataricus
1.1.1.47 Mg2+ maximal activity at 20 mM Saccharolobus solfataricus
1.1.1.47 Mn2+ maximal activity at 20 mM Saccharolobus solfataricus
1.1.1.47 Zn2+
-
 Saccharolobus solfataricus
1.1.1.118 Ca2+ maximally active at 20 mM Saccharolobus solfataricus
1.1.1.118 Mg2+ maximally active at 20 mM Saccharolobus solfataricus
1.1.1.118 Mn2+ maximally active at 20 mM Saccharolobus solfataricus
1.1.1.118 additional information Zn2+ is partially effective, whereas Ni2+, Cd2+ and univalent cations are ineffective in promoting enzyme reactivation Saccharolobus solfataricus
1.1.1.359 Ca2+ 20 mM, maximal activation of enzyme inactivated by dialysis against Mg2+-free 20 mM-triethanolamine/HCl buffer, pH 7.0 Saccharolobus solfataricus
1.1.1.359 Mg2+ 20 mM, maximal activation of enzyme inactivated by dialysis against Mg2+-free 20 mM-triethanolamine/HCl buffer, pH 7.0 Saccharolobus solfataricus
1.1.1.359 Mn2+ 20 mM, maximal activation of enzyme inactivated by dialysis against Mg2+-free 20 mM-triethanolamine/HCl buffer, pH 7.0 Saccharolobus solfataricus
1.1.1.359 additional information Ni2+, Cd2+ and univalent cations are ineffective in promoting enzyme reactivation Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.47 33000
-
 4 * 33000, SDS-PAGE Saccharolobus solfataricus
1.1.1.47 124000
-
 gel filtration Saccharolobus solfataricus
1.1.1.47 130000
-
 sedimentation equilibrium Saccharolobus solfataricus
1.1.1.118 30100
-
 4 * 30100, gel filtration, in the presence of 5% (w/v) SDS Saccharolobus solfataricus
1.1.1.118 60200
-
 2 * 60200, gel filtration, in the presence of 6 M guanidinium chloride Saccharolobus solfataricus
1.1.1.118 124000
-
 gel filtration Saccharolobus solfataricus
1.1.1.359 30000
-
 gel filtration in presence of 5% SDS Saccharolobus solfataricus
1.1.1.359 60000
-
 gel filtration in presence of 6 M guanidinium chloride Saccharolobus solfataricus
1.1.1.359 124000
-
 gel filtration Saccharolobus solfataricus
1.1.1.359 130000
-
 equilibrium-sedimentation Saccharolobus solfataricus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.1.1.47 Acetone 50%, 24 h, room temperature, no loss of activity Saccharolobus solfataricus
1.1.1.47 Ethanol 100%, slow decrease Saccharolobus solfataricus
1.1.1.47 Methanol 50%, 24 h, room temperature, no loss of activity Saccharolobus solfataricus
1.1.1.118 Acetone stable in the presence of chaotropic agents and water-miscible organic solvents such as acetone Saccharolobus solfataricus
1.1.1.118 Ethanol a slow time-dependent decrease of activity is observed in the presence of ethanol Saccharolobus solfataricus
1.1.1.118 Methanol stable in the presence of chaotropic agents and water-miscible organic solvents such as methanol Saccharolobus solfataricus
1.1.1.118 additional information 2 M urea and 0.05% (w/v) SDS do not cause any loss of enzymic activity Saccharolobus solfataricus
1.1.1.118 SDS a slow time-dependent decrease of activity is observed in the presence of 0.1% SDS Saccharolobus solfataricus
1.1.1.118 urea a slow time-dependent decrease of activity is observed in the presence of 4 M urea Saccharolobus solfataricus
1.1.1.359 Ethylene glycol preparations became inactivated irreversibly when stored in the absence of ethylene glycol Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.47 Saccharolobus solfataricus
-
-
-
1.1.1.118 Saccharolobus solfataricus
-
-
-
1.1.1.118 Saccharolobus solfataricus MT-4 / DSM 5833
-
-
-
1.1.1.359 Saccharolobus solfataricus O93715
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.47 ammonium sulfate, SP-Sephadex, Phenyl-Sepharose, Affi-Gel Blue Saccharolobus solfataricus
1.1.1.118 SP-Sephadex C-50 gel filtration, phenyl-Sepharose column chromatography, and Affi-Gel Blue matrix gel filtration Saccharolobus solfataricus
1.1.1.359
-
 Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.47 437
-
-
 Saccharolobus solfataricus
1.1.1.118 0.7
-
 unpurified enzyme, at 70°C Saccharolobus solfataricus
1.1.1.118 437
-
 after 623fold purification, at 70°C Saccharolobus solfataricus
1.1.1.359 437
-
 pH 9.0, 70°C Saccharolobus solfataricus

Storage Stability

EC Number Storage Stability Organism
1.1.1.47 4°C, 20 mM MgCl2, 20% ethylene glycol, several months, stable Saccharolobus solfataricus
1.1.1.118 37°C, in the presence of 20 mM MgCl2 and 20% (v/v) ethylene glycol, 40 days, 50% loss of activity Saccharolobus solfataricus
1.1.1.118 4°C, in the presence of 20 mM MgCl2 and 20% (v/v) ethylene glycol, several months, remains stable Saccharolobus solfataricus
1.1.1.118 70°C, in the presence of 20 mM MgCl2 and 20% (v/v) ethylene glycol, 45 h, 50% loss of activity Saccharolobus solfataricus
1.1.1.359 37°C, protein concentration 0.2 mg/ml, 50% of the activity is lost after 40 days Saccharolobus solfataricus
1.1.1.359 4°C, 20 mM MgCl2 and 20% (v/v) ethylene glycol, stable for several months Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.47 2-amino-2-deoxy-D-glucose + NAD+ 26% of the activity with D-glucose Saccharolobus solfataricus 2-amino-2-deoxy-D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.47 2-amino-2-deoxy-D-glucose + NADP+ 5% of the activity with D-glucose and NAD+ Saccharolobus solfataricus 2-amino-2-deoxy-D-glucono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.47 2-deoxy-D-glucose + NAD+ 12% of the activity with glucose Saccharolobus solfataricus 2-deoxy-D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.47 2-deoxy-D-glucose + NADP+ 25% of the activity with D-glucose and NAD+ Saccharolobus solfataricus 2-deoxy-D-glucono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.47 6-deoxy-D-glucose + NAD+ 66% of the activity with D-glucose Saccharolobus solfataricus 6-deoxy-D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.47 6-deoxy-D-glucose + NADP+ 9% of the activity with D-glucose and NAD+ Saccharolobus solfataricus ? + NADPH
-
 ?
1.1.1.47 beta-D-glucose + NAD+ 9% of the activity with NAD+ Saccharolobus solfataricus D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.47 beta-D-glucose + NADP+
-
 Saccharolobus solfataricus D-glucono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.47 beta-D-xylose + NAD+ 26% activity compared to D-glucose Saccharolobus solfataricus D-xylono-1,5-lactone + NADH + H+
-
 r
1.1.1.47 D-allose + NAD+ 8% of the activity with D-glucose Saccharolobus solfataricus D-allono-1,5-lactone + NADH
-
 ?
1.1.1.47 D-allose + NADP+ 13% of the activity with D-glucose and NAD+ Saccharolobus solfataricus D-allono-1,5-lactone + NADPH
-
 ?
1.1.1.47 D-altrose + NAD+ 5% of the activity with D-glucose Saccharolobus solfataricus D-altrono-1,5-lactone + NADH
-
 ?
1.1.1.47 D-altrose + NADP+ 12% of the activity with D-glucose and NAD+ Saccharolobus solfataricus D-altrono-1,5-lactone + NADPH
-
 ?
1.1.1.47 D-galactose + NAD+ 15% of the activity with D-glucose and NAD+ Saccharolobus solfataricus D-galactono-1,5-lactone + NADH
-
 ?
1.1.1.47 D-gulose + NAD+ 8% of the activity with D-glucose Saccharolobus solfataricus D-gulono-1,5-lactone + NADH
-
 ?
1.1.1.47 D-gulose + NADP+ 12% of the activity with D-glucose and NAD+ Saccharolobus solfataricus D-gulono-1,5-lactone + NADPH
-
 ?
1.1.1.47 D-idose + NAD+ 65% of the activity with D-glucose Saccharolobus solfataricus D-idono-1,5-lactone + NADH
-
 ?
1.1.1.47 D-idose + NADP+ 12% of the activity with D-glucose and NAD+ Saccharolobus solfataricus D-idono-1,5-lactone + NADPH
-
 ?
1.1.1.47 D-mannose + NADP+ no activity Saccharolobus solfataricus D-mannono-1,5-lactone + NADPH
-
 ?
1.1.1.47 D-ribose + NADP+ 4% of the activity with D-glucose and NADP+ Saccharolobus solfataricus D-ribono-1,5-lactone + NADPH
-
 ?
1.1.1.47 D-xylose + NAD+ 28% of the activity with D-glucose and NAD+ Saccharolobus solfataricus D-xylono-1,5-lactone + NADH + H+
-
 ?
1.1.1.47 D-xylose + NADP+ 28% of the activity with D-glucose and NAD+ Saccharolobus solfataricus D-xylono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.118 2-amino-2-deoxy-D-glucose + NAD+ 26% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus ? + NADH
-
 ?
1.1.1.118 2-amino-2-deoxy-D-glucose + NADP+ 5% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus ? + NADPH
-
 ?
1.1.1.118 2-deoxy-D-glucose + NAD+ 12% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus 2-deoxy-D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.118 2-deoxy-D-glucose + NAD+ 12% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus MT-4 / DSM 5833 2-deoxy-D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.118 2-deoxy-D-glucose + NADP+ 25% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus ? + NADPH
-
 ?
1.1.1.118 6-deoxy-D-glucose + NAD+ 66% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus 6-deoxy-D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.118 6-deoxy-D-glucose + NAD+ 66% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus MT-4 / DSM 5833 6-deoxy-D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.118 6-deoxy-D-glucose + NADP+ 9% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus ? + NADPH
-
 ?
1.1.1.118 D-altrose + NAD+ 5% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus D-altrono-1,5-lactone + NADH + H+
-
 ?
1.1.1.118 D-altrose + NADP+ 12% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus ? + NADPH
-
 ?
1.1.1.118 D-galactose + NAD+ 15% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus D-galactono-1,5-lactone + NADH + H+
-
 ?
1.1.1.118 D-glucose + NAD+ 100% activity Saccharolobus solfataricus D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.118 D-glucose + NAD+ 100% activity Saccharolobus solfataricus MT-4 / DSM 5833 D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.118 D-glucose + NADP+ 9% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus D-glucono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.118 D-glucose + NADP+ 9% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus MT-4 / DSM 5833 D-glucono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.118 D-gulose + NAD+ 8% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus ? + NADH
-
 ?
1.1.1.118 D-gulose + NADP+ 12% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus ? + NADPH
-
 ?
1.1.1.118 D-idose + NAD+ 65% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus ? + NADH
-
 ?
1.1.1.118 D-idose + NADP+ 12% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus ? + NADPH
-
 ?
1.1.1.118 D-mannose + NADP+ 4% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus ? + NADPH
-
 ?
1.1.1.118 D-ribose + NADP+ 4% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus ? + NADPH
-
 ?
1.1.1.118 D-xylose + NAD+ 26% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus ? + NADH
-
 ?
1.1.1.118 D-xylose + NADP+ 28% activity compared to D-glucose in combination with NAD+ Saccharolobus solfataricus ? + NADPH
-
 ?
1.1.1.118 additional information no activity towards D-mannose,D-galactose, and D-ribose in combination with NAD+ Saccharolobus solfataricus ?
-
 ?
1.1.1.118 additional information no activity towards D-mannose,D-galactose, and D-ribose in combination with NAD+ Saccharolobus solfataricus MT-4 / DSM 5833 ?
-
 ?
1.1.1.359 2-deoxy-D-glucose + NADP+ + H2O
-
 Saccharolobus solfataricus ?
-
 ?
1.1.1.359 6-deoxy-D-glucose + NAD+ + H2O
-
 Saccharolobus solfataricus ?
-
 ?
1.1.1.359 beta-D-glucose + NAD+ + H2O when NAD+ serves as electron acceptor, D-glucose is oxidized at the highest rate among the substrates tested. No reverse reaction is evident, under various reaction conditions, when gluconic acid is used as substrate Saccharolobus solfataricus D-gluconate + NADH + 2 H+
-
 ir
1.1.1.359 beta-D-glucose + NADP+ + H2O it is likely that the first product of this reaction is delta-gluconolactone, which is readily converted into the acid. The lactone hydrolysis is facilitated by the high temperature and the slight alkaline pH value of the standard reaction conditions, thus explaining why no reverse reaction is observed under these conditions when delta-gluconolactone is used as substrate. A very slow NADH-dependent reduction of delta-gluconolactone by glucose dehydrogenase is observed at pH 7.0 and 37°C Saccharolobus solfataricus D-gluconate + NADPH + 2 H+
-
 ?
1.1.1.359 D-altrose + NADP+ + H2O
-
 Saccharolobus solfataricus ?
-
 ?
1.1.1.359 D-galactose + NADP+ + H2O
-
 Saccharolobus solfataricus D-galactonate + NADPH + 2 H+
-
 ?
1.1.1.359 D-xylose + NAD+ + H2O
-
 Saccharolobus solfataricus ?
-
 ?
1.1.1.359 D-xylose + NADP+ + H2O
-
 Saccharolobus solfataricus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.47 tetramer 4 * 33000, SDS-PAGE Saccharolobus solfataricus
1.1.1.118 homodimer 2 * 60200, gel filtration, in the presence of 6 M guanidinium chloride Saccharolobus solfataricus
1.1.1.118 homotetramer 4 * 30100, gel filtration, in the presence of 5% (w/v) SDS Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.118 glucose dehydrogenase
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.47 77
-
 at pH 8.0 Saccharolobus solfataricus
1.1.1.118 77
-
-
 Saccharolobus solfataricus
1.1.1.359 70
-
 assay at Saccharolobus solfataricus
1.1.1.359 77
-
-
 Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.47 37 77 37°C: 20% of maximal activity, 77°C: optimum Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.359 37
-
 protein concentration 0.2 mg/ml, 50% of the activity is lost after 40 days Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.47 8
-
-
 Saccharolobus solfataricus
1.1.1.118 8
-
-
 Saccharolobus solfataricus
1.1.1.359 8
-
-
 Saccharolobus solfataricus
1.1.1.359 9
-
 assay at, measured at room temperature Saccharolobus solfataricus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.118 5 9 the enzyme stability does not vary significantly in the pH range 5-9 Saccharolobus solfataricus
1.1.1.359 5 9 the enzyme stability does not vary significantly in the pH range 5-9 Saccharolobus solfataricus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.47 NAD+
-
 Saccharolobus solfataricus
1.1.1.47 NADP+
-
 Saccharolobus solfataricus
1.1.1.118 NAD+
-
 Saccharolobus solfataricus
1.1.1.118 NADP+
-
 Saccharolobus solfataricus
1.1.1.359 NAD+ when NAD+ serves as electron acceptor, D-glucose is oxidized at the highest rate among the substrates tested Saccharolobus solfataricus
1.1.1.359 NADP+ by using NADP+ as coenzyme D-xylose and 2-deoxy-D-glucose are oxidized at the highest rate Saccharolobus solfataricus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.118 0.075
-
 NADPH using D-glucose as a substrate Saccharolobus solfataricus
1.1.1.359 0.075
-
 NADPH pH 9.0, 70°C, substrate: glucose Saccharolobus solfataricus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.1.1.118 1
-
 at 25°C and pH 9 Saccharolobus solfataricus N-ethylmaleimide
1.1.1.118 3
-
 at 25°C and pH 9 Saccharolobus solfataricus 5,5'-dithiobis-(2-nitrobenzoic acid)