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Literature summary extracted from

  • Bhaumik, S.R.; Sonawat, H.M.
    Kinetic mechanism of glucose dehydrogenase from Halobacterium salinarum (1999), Indian J. Biochem. Biophys., 36, 143-149.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.47 D-glucono-1,5-lactone forward reaction, oxidation, product inhibition, noncompetitive vs. beta-D-glucose and NADP+ Halobacterium salinarum
1.1.1.47 NADP+ reverse reaction, reduction, product inhibition, competitive vs. NADPH and noncompetitive vs. D-glucono-1-5-lactone Halobacterium salinarum
1.1.1.47 NADPH product inhibition, competitive with respect to NADP Halobacterium salinarum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.47 0.14
-
 beta-D-glucose reduction, reverse reaction Halobacterium salinarum
1.1.1.47 0.19
-
 NADP+
-
 Halobacterium salinarum
1.1.1.47 0.49
-
 NADPH
-
 Halobacterium salinarum
1.1.1.47 2.57
-
 beta-D-glucose oxidation, forward reaction Halobacterium salinarum

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.47 Halobacterium salinarum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.47 ammonium sulfate, Sepharose 6B, DEAE-Sepharose, hydroxyapatite chromatography Halobacterium salinarum

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.47 D-glucose + NAD(P)+ = D-glucono-1,5-lactone + NAD(P)H + H+ ordered bi bi mechanism, formation of ternary complexes, NADP+ adds first, NADPH formed dissociates last, for reverse reaction, NADPH adds first, NADP+ leaves last Halobacterium salinarum
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.47 beta-D-glucose + NADP+
-
 Halobacterium salinarum D-glucono-1,5-lactone + NADPH + H+
-
 r

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.47 NADP+
-
 Halobacterium salinarum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.47 additional information
-
 additional information
-
 Halobacterium salinarum