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Literature summary extracted from

  • Heidlas, J.; Engel, K.H.; Tressl, R.
    Purification and characterization of two oxidoreductases involved in the enantioselective reduction of 3-oxo, 4-oxo and 5-oxo esters in bakers yeast (1988), Eur. J. Biochem., 172, 633-639.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.279 8-hydroxyquinoline 5-sulfonic acid
-
 Saccharomyces cerevisiae
1.1.1.279 HgCl2
-
 Saccharomyces cerevisiae
1.1.1.279 o-phenanthroline
-
 Saccharomyces cerevisiae
1.1.1.280 8-hydroxyquinoline 5-sulfonic acid
-
 Saccharomyces cerevisiae
1.1.1.280 dipicolinate
-
 Saccharomyces cerevisiae
1.1.1.280 HgCl2
-
 Saccharomyces cerevisiae
1.1.1.280 o-phenanthroline
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.279 2
-
 ethyl 3-oxohexanoate
-
 Saccharomyces cerevisiae
1.1.1.279 17
-
 ethyl 3-oxobutanoate
-
 Saccharomyces cerevisiae
1.1.1.280 0.9
-
 ethyl 3-oxobutyrate
-
 Saccharomyces cerevisiae
1.1.1.280 5.3
-
 ethyl 3-oxohexanoate
-
 Saccharomyces cerevisiae
1.1.1.280 13.1
-
 5-Oxohexanoate
-
 Saccharomyces cerevisiae
1.1.1.280 17.1
-
 4-oxopentanoate
-
 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.279 cytosol
-
 Saccharomyces cerevisiae 5829
-
1.1.1.280 cytosol
-
 Saccharomyces cerevisiae 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.279 200000
-
 x * 210000 + x * 200000, SDS-PAGE Saccharomyces cerevisiae
1.1.1.279 210000
-
 x * 210000 + x * 200000, SDS-PAGE Saccharomyces cerevisiae
1.1.1.279 780000 800000 gel filtration Saccharomyces cerevisiae
1.1.1.280 24000
-
 2 * 24000, SDS-PAGE Saccharomyces cerevisiae
1.1.1.280 48000 50000 gel filtration Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.279 Saccharomyces cerevisiae
-
-
-
1.1.1.280 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.279 using streptomycin sulfate treatment, Sephadex G-25 filtration, DEAE-Sepharose CL-6B chromatography, Sephadex G-150 filtration, Sepharose 6B filtration and hydroxyapatite chromatography Saccharomyces cerevisiae
1.1.1.280 using streptomycin sulfate treatment, Sephadex G-25 filtration, DEAE-Sepharose CL-6B chromatography, Sephadex G-150 filtration, Sepharose 6B filtration and hydroxyapatite chromatography Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.279 1.958
-
-
 Saccharomyces cerevisiae
1.1.1.280 0.411
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.279 ethyl 3-oxobutanoate + NADPH
-
 Saccharomyces cerevisiae ethyl (R)-3-hydroxybutanoate + NADP+
-
 ?
1.1.1.279 ethyl 3-oxohexanoate + NADPH
-
 Saccharomyces cerevisiae ethyl (R)-3-hydroxyhexanoate + NADP+
-
 ?
1.1.1.279 additional information specific for 3-oxo-esters, no reaction with 3-oxo-acids Saccharomyces cerevisiae ?
-
 ?
1.1.1.280 4-oxopentanoate + NADPH
-
 Saccharomyces cerevisiae (S)-4-hydroxypentanoate + NADP+
-
 ?
1.1.1.280 5-oxohexanoate + NADPH
-
 Saccharomyces cerevisiae (S)-5-hydroxyhexanoate + NADP+
-
 ?
1.1.1.280 ethyl 3-oxobutyrate + NADPH
-
 Saccharomyces cerevisiae ethyl (S)-3-hydroxybutyrate + NADP+
-
 ?
1.1.1.280 ethyl 3-oxohexanoate + NADPH
-
 Saccharomyces cerevisiae ethyl (S)-3-hydroxyhexanoate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.279 oligomer x * 210000 + x * 200000, SDS-PAGE Saccharomyces cerevisiae
1.1.1.280 dimer 2 * 24000, SDS-PAGE Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.279 6.1
-
-
 Saccharomyces cerevisiae
1.1.1.280 6.9
-
-
 Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.279 7.5
-
 rapid decrease of activity above Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.279 NADPH specific for Saccharomyces cerevisiae
1.1.1.280 NADPH specific for Saccharomyces cerevisiae