BRENDA - Enzyme Database

A novel NADP+ dependent serine dehydrogenase from Agrobacterium tumefaciens

Chowdhury, E.K.; Higuchi, K.; Nagata, S.; Misono, H.; Biosci. Biotechnol. Biochem. 61, 152-157 (1997)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.276
2-methylmalonate
competitive against L-serine, noncompetitive against NADP+
Agrobacterium tumefaciens
1.1.1.276
Co2+
weak
Agrobacterium tumefaciens
1.1.1.276
D-cysteine
competitive against L-serine, noncompetitive against NADP+
Agrobacterium tumefaciens
1.1.1.276
Hg2+
strong
Agrobacterium tumefaciens
1.1.1.276
L-cysteine
competitive against L-serine, noncompetitive against NADP+
Agrobacterium tumefaciens
1.1.1.276
malonate
competitive against L-serine, noncompetitive against NADP+
Agrobacterium tumefaciens
1.1.1.276
additional information
no inhibition by EDTA, sodium arsenate, o-phenethroline, alpha,alpha'-dipyridyl and very slight inhibition by sodium azide
Agrobacterium tumefaciens
1.1.1.276
N-ethylmaleimide
-
Agrobacterium tumefaciens
1.1.1.276
p-chloromercuribenzoate
strong
Agrobacterium tumefaciens
1.1.1.276
Tartronate
competitive against L-serine, noncompetitive against NADP+
Agrobacterium tumefaciens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.276
0.029
-
NADP+
pH 9.0, 30C
Agrobacterium tumefaciens
1.1.1.276
42
-
L-serine
pH 9.0, 30 C
Agrobacterium tumefaciens
1.1.1.276
44
-
D-serine
pH 9.0, 30 C
Agrobacterium tumefaciens
1.1.1.276
54
-
L-Glycerate
pH 9.0, 30 C
Agrobacterium tumefaciens
1.1.1.276
56
-
D-glycerate
pH 9.0, 30 C
Agrobacterium tumefaciens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.276
additional information
no effect with Cu2+, Mg2+, Mn2+, Zn2+, Fe2+
Agrobacterium tumefaciens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.276
100000
-
gel filtration
Agrobacterium tumefaciens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.276
L-serine + NADP+
Agrobacterium tumefaciens
-
2-aminomalonate semialdehyde + NADPH
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.276
Agrobacterium tumefaciens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.276
-
Agrobacterium tumefaciens
Reaction
EC Number
Reaction
Commentary
Organism
1.1.1.276
L-serine + NADP+ = 2-aminoacetaldehyde + CO2 + NADPH + H+
sequential mechanism
Agrobacterium tumefaciens
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.1.1.276
45.3
-
purified enzyme
Agrobacterium tumefaciens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.276
2-methyl-DL-serine + NADP+
8.7% activity compared to L-serine
639082
Agrobacterium tumefaciens
2-amino-2-methyl-3-oxopropanoic acid + NADPH
-
-
-
?
1.1.1.276
3-hydroxypropanoate + NADP+
1.6% activity compared to L-serine
639082
Agrobacterium tumefaciens
3-oxopropanoate + NADPH
-
-
-
?
1.1.1.276
D-3-hydroxyisobutyrate + NADP+
3.5% activity compared to L-serine
639082
Agrobacterium tumefaciens
2-methyl-3-oxopropanoic acid + NADPH
-
-
-
?
1.1.1.276
D-glycerate + NADP+
12.9% activity compared to L-serine
639082
Agrobacterium tumefaciens
?
-
-
-
?
1.1.1.276
D-serine + NADP+
55.2% activity compared to L-serine
639082
Agrobacterium tumefaciens
2-aminomalonate semialdehyde + NADPH
-
-
-
?
1.1.1.276
L-3-hydroxyisobutyrate + NADP+
8.5% activity compared to L-serine
639082
Agrobacterium tumefaciens
2-methyl-3-oxopropanoic acid + NADPH
-
-
-
?
1.1.1.276
L-glycerate + NADP+
11.3% activity compared to L-serine
639082
Agrobacterium tumefaciens
?
-
-
-
?
1.1.1.276
L-serine + NADP+
-
639082
Agrobacterium tumefaciens
2-aminomalonate semialdehyde + NADPH
-
-
-
?
1.1.1.276
L-serine + NADP+
best substrate
639082
Agrobacterium tumefaciens
2-aminomalonate semialdehyde + NADPH
-
-
-
?
1.1.1.276
additional information
no activity with 4-hydroxybutyrate, DL-2-hydroxybutyrate, DL-2-hydroxyisobutyrate, DL-2-hydroxycaproate, DL-2-hydroxyisocaproate, 3-phosphoglycerate, DL-lactate, L-malate, glycollate, glycerol, D-threonine, DL-threo-phenylserine, DL-homoserine, DL-isoserine, L-alanine, beta-alanine, L-aspartate, L-glutamate, L-valine, L-leucine, L-methionine, L-lysine, L-phenylalanine, and glycine
639082
Agrobacterium tumefaciens
?
-
-
-
-
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.1.1.276
30
-
assay at
Agrobacterium tumefaciens
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.1.1.276
35
-
stable at
Agrobacterium tumefaciens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.276
9.1
-
-
Agrobacterium tumefaciens
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.1.276
7
8
stable at
Agrobacterium tumefaciens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.276
NADP+
dependent on; NAD+ cannot replace NADP+
Agrobacterium tumefaciens
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.1.1.276
0.067
-
L-cysteine
versus L-serine, pH 9.0, 30C
Agrobacterium tumefaciens
1.1.1.276
0.21
-
L-cysteine
versus NADP+, pH 9.0, 30C
Agrobacterium tumefaciens
1.1.1.276
0.59
-
D-cysteine
versus L-serine, pH 9.0, 30C
Agrobacterium tumefaciens
1.1.1.276
9.9
-
D-cysteine
versus NADP+, pH 9.0, 30C
Agrobacterium tumefaciens
1.1.1.276
10
-
2-methylmalonate
versus NADP+, pH 9.0, 30C
Agrobacterium tumefaciens
1.1.1.276
11
-
malonate
versus L-serine, pH 9.0, 30C
Agrobacterium tumefaciens
1.1.1.276
12
-
2-methylmalonate
versus L-serine, pH 9.0, 30C
Agrobacterium tumefaciens
1.1.1.276
13
-
Tartronate
versus L-serine, pH 9.0, 30C
Agrobacterium tumefaciens
1.1.1.276
17
-
malonate
versus NADP+, pH 9.0, 30C
Agrobacterium tumefaciens
1.1.1.276
18
-
Tartronate
versus NADP+, pH 9.0, 30C
Agrobacterium tumefaciens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.276
NADP+
dependent on; NAD+ cannot replace NADP+
Agrobacterium tumefaciens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.276
2-methylmalonate
competitive against L-serine, noncompetitive against NADP+
Agrobacterium tumefaciens
1.1.1.276
Co2+
weak
Agrobacterium tumefaciens
1.1.1.276
D-cysteine
competitive against L-serine, noncompetitive against NADP+
Agrobacterium tumefaciens
1.1.1.276
Hg2+
strong
Agrobacterium tumefaciens
1.1.1.276
L-cysteine
competitive against L-serine, noncompetitive against NADP+
Agrobacterium tumefaciens
1.1.1.276
malonate
competitive against L-serine, noncompetitive against NADP+
Agrobacterium tumefaciens
1.1.1.276
additional information
no inhibition by EDTA, sodium arsenate, o-phenethroline, alpha,alpha'-dipyridyl and very slight inhibition by sodium azide
Agrobacterium tumefaciens
1.1.1.276
N-ethylmaleimide
-
Agrobacterium tumefaciens
1.1.1.276
p-chloromercuribenzoate
strong
Agrobacterium tumefaciens
1.1.1.276
Tartronate
competitive against L-serine, noncompetitive against NADP+
Agrobacterium tumefaciens
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.1.1.276
0.067
-
L-cysteine
versus L-serine, pH 9.0, 30C
Agrobacterium tumefaciens
1.1.1.276
0.21
-
L-cysteine
versus NADP+, pH 9.0, 30C
Agrobacterium tumefaciens
1.1.1.276
0.59
-
D-cysteine
versus L-serine, pH 9.0, 30C
Agrobacterium tumefaciens
1.1.1.276
9.9
-
D-cysteine
versus NADP+, pH 9.0, 30C
Agrobacterium tumefaciens
1.1.1.276
10
-
2-methylmalonate
versus NADP+, pH 9.0, 30C
Agrobacterium tumefaciens
1.1.1.276
11
-
malonate
versus L-serine, pH 9.0, 30C
Agrobacterium tumefaciens
1.1.1.276
12
-
2-methylmalonate
versus L-serine, pH 9.0, 30C
Agrobacterium tumefaciens
1.1.1.276
13
-
Tartronate
versus L-serine, pH 9.0, 30C
Agrobacterium tumefaciens
1.1.1.276
17
-
malonate
versus NADP+, pH 9.0, 30C
Agrobacterium tumefaciens
1.1.1.276
18
-
Tartronate
versus NADP+, pH 9.0, 30C
Agrobacterium tumefaciens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.276
0.029
-
NADP+
pH 9.0, 30C
Agrobacterium tumefaciens
1.1.1.276
42
-
L-serine
pH 9.0, 30 C
Agrobacterium tumefaciens
1.1.1.276
44
-
D-serine
pH 9.0, 30 C
Agrobacterium tumefaciens
1.1.1.276
54
-
L-Glycerate
pH 9.0, 30 C
Agrobacterium tumefaciens
1.1.1.276
56
-
D-glycerate
pH 9.0, 30 C
Agrobacterium tumefaciens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.276
additional information
no effect with Cu2+, Mg2+, Mn2+, Zn2+, Fe2+
Agrobacterium tumefaciens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.276
100000
-
gel filtration
Agrobacterium tumefaciens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.276
L-serine + NADP+
Agrobacterium tumefaciens
-
2-aminomalonate semialdehyde + NADPH
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.276
-
Agrobacterium tumefaciens
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.1.1.276
45.3
-
purified enzyme
Agrobacterium tumefaciens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.276
2-methyl-DL-serine + NADP+
8.7% activity compared to L-serine
639082
Agrobacterium tumefaciens
2-amino-2-methyl-3-oxopropanoic acid + NADPH
-
-
-
?
1.1.1.276
3-hydroxypropanoate + NADP+
1.6% activity compared to L-serine
639082
Agrobacterium tumefaciens
3-oxopropanoate + NADPH
-
-
-
?
1.1.1.276
D-3-hydroxyisobutyrate + NADP+
3.5% activity compared to L-serine
639082
Agrobacterium tumefaciens
2-methyl-3-oxopropanoic acid + NADPH
-
-
-
?
1.1.1.276
D-glycerate + NADP+
12.9% activity compared to L-serine
639082
Agrobacterium tumefaciens
?
-
-
-
?
1.1.1.276
D-serine + NADP+
55.2% activity compared to L-serine
639082
Agrobacterium tumefaciens
2-aminomalonate semialdehyde + NADPH
-
-
-
?
1.1.1.276
L-3-hydroxyisobutyrate + NADP+
8.5% activity compared to L-serine
639082
Agrobacterium tumefaciens
2-methyl-3-oxopropanoic acid + NADPH
-
-
-
?
1.1.1.276
L-glycerate + NADP+
11.3% activity compared to L-serine
639082
Agrobacterium tumefaciens
?
-
-
-
?
1.1.1.276
L-serine + NADP+
-
639082
Agrobacterium tumefaciens
2-aminomalonate semialdehyde + NADPH
-
-
-
?
1.1.1.276
L-serine + NADP+
best substrate
639082
Agrobacterium tumefaciens
2-aminomalonate semialdehyde + NADPH
-
-
-
?
1.1.1.276
additional information
no activity with 4-hydroxybutyrate, DL-2-hydroxybutyrate, DL-2-hydroxyisobutyrate, DL-2-hydroxycaproate, DL-2-hydroxyisocaproate, 3-phosphoglycerate, DL-lactate, L-malate, glycollate, glycerol, D-threonine, DL-threo-phenylserine, DL-homoserine, DL-isoserine, L-alanine, beta-alanine, L-aspartate, L-glutamate, L-valine, L-leucine, L-methionine, L-lysine, L-phenylalanine, and glycine
639082
Agrobacterium tumefaciens
?
-
-
-
-
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.1.1.276
30
-
assay at
Agrobacterium tumefaciens
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.1.1.276
35
-
stable at
Agrobacterium tumefaciens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.276
9.1
-
-
Agrobacterium tumefaciens
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.1.276
7
8
stable at
Agrobacterium tumefaciens