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Literature summary extracted from

  • Lu, Z.J.; Markham, G.D.
    Enzymatic properties of S-adenosylmethionine synthetase from the archaeon Methanococcus jannaschii (2002), J. Biol. Chem., 277, 16624-16631.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.6 expressed in Escherichia coli BL21(DE3)codon plus/pMJ1208-strain with a decahistidine tag on the N-terminus Methanocaldococcus jannaschii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.6 GTP competitive with respect to ATP and noncompetitive with L-methionine Methanocaldococcus jannaschii
2.5.1.6 L-ethionine competitive with respect to methionine for S-adenosylmethionine formation and noncompetitive with respect to ATP Methanocaldococcus jannaschii
2.5.1.6 additional information no inhibition with cycloleucine, L-homocysteine, L-norleucine, L-cis-2-amino-4-methoxy-3-butenoic acid, S-adenosylhomocysteine, 5'-methylthioadenosine, sinefungin Methanocaldococcus jannaschii
2.5.1.6 phosphate competitive toward both ATP and methionine Methanocaldococcus jannaschii
2.5.1.6 S-adenosylmethionine noncompetitive inhibitor with respect to ATP and methionine Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.6 0.22
-
ATP saturated with KCl Methanocaldococcus jannaschii
2.5.1.6 0.24
-
L-methionine
-
Methanocaldococcus jannaschii
2.5.1.6 0.26
-
ATP
-
Methanocaldococcus jannaschii
2.5.1.6 0.3
-
L-methionine saturated with KCl Methanocaldococcus jannaschii
2.5.1.6 0.4
-
CTP
-
Methanocaldococcus jannaschii
2.5.1.6 0.62
-
GTP
-
Methanocaldococcus jannaschii
2.5.1.6 0.74
-
L-ethionine
-
Methanocaldococcus jannaschii
2.5.1.6 1.1
-
L-methionine
-
Methanocaldococcus jannaschii
2.5.1.6 1.4
-
ATP
-
Methanocaldococcus jannaschii
2.5.1.6 2.2
-
UTP
-
Methanocaldococcus jannaschii
2.5.1.6 2.6
-
L-methionine methyl esther
-
Methanocaldococcus jannaschii
2.5.1.6 3.5
-
D-methionine
-
Methanocaldococcus jannaschii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.6 K+ enhances Kcat and decrees Km values for both substrates Methanocaldococcus jannaschii
2.5.1.6 Mg2+ absolute requirement Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.6 45000
-
2 * 45000 Methanocaldococcus jannaschii
2.5.1.6 86000
-
recombinant enzyme, gel filtration Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.6 ATP + L-methionine + H2O Methanocaldococcus jannaschii
-
S-adenosyl-L-methionine + phosphate + diphosphate
-
ir

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.6 Methanocaldococcus jannaschii
-
hypertermophilic archaeon
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.6 recombinant enzyme purified by a method that includes Ni2+-His binding resin, HiLoad Q-Sepharose and Sephadex-200 chromatography Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.6 2'-deoxy-ATP + L-methionine + H2O
-
Methanocaldococcus jannaschii ?
-
ir
2.5.1.6 3'-deoxy-ATP + L-methionine + H2O
-
Methanocaldococcus jannaschii ?
-
ir
2.5.1.6 ATP + D-methionine + H2O
-
Methanocaldococcus jannaschii S-adenosyl-D-methionine + phosphate + diphosphate
-
?
2.5.1.6 ATP + L-ethionine + H2O
-
Methanocaldococcus jannaschii S-adenosyl-L-ethionine + phosphate + diphosphate
-
?
2.5.1.6 ATP + L-methionine + H2O
-
Methanocaldococcus jannaschii S-adenosyl-L-methionine + phosphate + diphosphate
-
ir
2.5.1.6 ATP + L-methionine methyl ester + H2O
-
Methanocaldococcus jannaschii S-adenosyl-L-methionine methyl ester + phosphate + diphosphate
-
?
2.5.1.6 CTP + L-methionine + H2O
-
Methanocaldococcus jannaschii S-cytosyl-L-methionine + phosphate + diphosphate
-
?
2.5.1.6 GTP + L-methionine + H2O
-
Methanocaldococcus jannaschii S-guanosyl-L-methionine + phosphate + diphosphate
-
?
2.5.1.6 additional information no activity with methional, methioninol, 3-methylthiopropylamine Methanocaldococcus jannaschii ?
-
?
2.5.1.6 UTP + L-methionine + H2O
-
Methanocaldococcus jannaschii S-urasyl-L-methionine + phosphate + diphosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.5.1.6 dimer 2 * 45000 Methanocaldococcus jannaschii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.6 70
-
optimal growth temperature for Methanococcus jannaschii: 87°C Methanocaldococcus jannaschii