EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.6 | expressed in Escherichia coli BL21(DE3)codon plus/pMJ1208-strain with a decahistidine tag on the N-terminus | Methanocaldococcus jannaschii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.6 | GTP | competitive with respect to ATP and noncompetitive with L-methionine | Methanocaldococcus jannaschii | |
2.5.1.6 | L-ethionine | competitive with respect to methionine for S-adenosylmethionine formation and noncompetitive with respect to ATP | Methanocaldococcus jannaschii | |
2.5.1.6 | additional information | no inhibition with cycloleucine, L-homocysteine, L-norleucine, L-cis-2-amino-4-methoxy-3-butenoic acid, S-adenosylhomocysteine, 5'-methylthioadenosine, sinefungin | Methanocaldococcus jannaschii | |
2.5.1.6 | phosphate | competitive toward both ATP and methionine | Methanocaldococcus jannaschii | |
2.5.1.6 | S-adenosylmethionine | noncompetitive inhibitor with respect to ATP and methionine | Methanocaldococcus jannaschii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.6 | 0.22 | - |
ATP | saturated with KCl | Methanocaldococcus jannaschii | |
2.5.1.6 | 0.24 | - |
L-methionine | - |
Methanocaldococcus jannaschii | |
2.5.1.6 | 0.26 | - |
ATP | - |
Methanocaldococcus jannaschii | |
2.5.1.6 | 0.3 | - |
L-methionine | saturated with KCl | Methanocaldococcus jannaschii | |
2.5.1.6 | 0.4 | - |
CTP | - |
Methanocaldococcus jannaschii | |
2.5.1.6 | 0.62 | - |
GTP | - |
Methanocaldococcus jannaschii | |
2.5.1.6 | 0.74 | - |
L-ethionine | - |
Methanocaldococcus jannaschii | |
2.5.1.6 | 1.1 | - |
L-methionine | - |
Methanocaldococcus jannaschii | |
2.5.1.6 | 1.4 | - |
ATP | - |
Methanocaldococcus jannaschii | |
2.5.1.6 | 2.2 | - |
UTP | - |
Methanocaldococcus jannaschii | |
2.5.1.6 | 2.6 | - |
L-methionine methyl esther | - |
Methanocaldococcus jannaschii | |
2.5.1.6 | 3.5 | - |
D-methionine | - |
Methanocaldococcus jannaschii |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.6 | K+ | enhances Kcat and decrees Km values for both substrates | Methanocaldococcus jannaschii | |
2.5.1.6 | Mg2+ | absolute requirement | Methanocaldococcus jannaschii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.6 | 45000 | - |
2 * 45000 | Methanocaldococcus jannaschii |
2.5.1.6 | 86000 | - |
recombinant enzyme, gel filtration | Methanocaldococcus jannaschii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.6 | ATP + L-methionine + H2O | Methanocaldococcus jannaschii | - |
S-adenosyl-L-methionine + phosphate + diphosphate | - |
ir |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.6 | Methanocaldococcus jannaschii | - |
hypertermophilic archaeon | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.6 | recombinant enzyme purified by a method that includes Ni2+-His binding resin, HiLoad Q-Sepharose and Sephadex-200 chromatography | Methanocaldococcus jannaschii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.6 | 2'-deoxy-ATP + L-methionine + H2O | - |
Methanocaldococcus jannaschii | ? | - |
ir | |
2.5.1.6 | 3'-deoxy-ATP + L-methionine + H2O | - |
Methanocaldococcus jannaschii | ? | - |
ir | |
2.5.1.6 | ATP + D-methionine + H2O | - |
Methanocaldococcus jannaschii | S-adenosyl-D-methionine + phosphate + diphosphate | - |
? | |
2.5.1.6 | ATP + L-ethionine + H2O | - |
Methanocaldococcus jannaschii | S-adenosyl-L-ethionine + phosphate + diphosphate | - |
? | |
2.5.1.6 | ATP + L-methionine + H2O | - |
Methanocaldococcus jannaschii | S-adenosyl-L-methionine + phosphate + diphosphate | - |
ir | |
2.5.1.6 | ATP + L-methionine methyl ester + H2O | - |
Methanocaldococcus jannaschii | S-adenosyl-L-methionine methyl ester + phosphate + diphosphate | - |
? | |
2.5.1.6 | CTP + L-methionine + H2O | - |
Methanocaldococcus jannaschii | S-cytosyl-L-methionine + phosphate + diphosphate | - |
? | |
2.5.1.6 | GTP + L-methionine + H2O | - |
Methanocaldococcus jannaschii | S-guanosyl-L-methionine + phosphate + diphosphate | - |
? | |
2.5.1.6 | additional information | no activity with methional, methioninol, 3-methylthiopropylamine | Methanocaldococcus jannaschii | ? | - |
? | |
2.5.1.6 | UTP + L-methionine + H2O | - |
Methanocaldococcus jannaschii | S-urasyl-L-methionine + phosphate + diphosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.6 | dimer | 2 * 45000 | Methanocaldococcus jannaschii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.6 | 70 | - |
optimal growth temperature for Methanococcus jannaschii: 87°C | Methanocaldococcus jannaschii |