EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.6 | three isoenzymes expressed in Escherichia coli | Catharanthus roseus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.6 | 5-amino-L-norvaline | 10-25% inhibition with 5 mM; 10-25% inhibition with 5 mM; 10-25% inhibition with 5 mM | Catharanthus roseus | |
2.5.1.6 | ADP | 35-50% inhibition with 5 mM; 35-50% inhibition with 5 mM; 35-50% inhibition with 5 mM | Catharanthus roseus | |
2.5.1.6 | CTP | 60-70% inhibition with 5 mM; 60-70% inhibition with 5 mM; 60-70% inhibition with 5 mM | Catharanthus roseus | |
2.5.1.6 | Ethionine | 32-38% inhibition with 5 mM; 32-38% inhibition with 5 mM; 32-38% inhibition with 5 mM | Catharanthus roseus | |
2.5.1.6 | GTP | not accepted as a substrate but inhibits the reaction in the presence of ATP, 70-80% inhibition with 5 mM; not accepted as a substrate but inhibits the reaction in the presence of ATP, 70-80% inhibition with 5 mM; not accepted as a substrate but inhibits the reaction in the presence of ATP, 70-80% inhibition with 5 mM | Catharanthus roseus | |
2.5.1.6 | additional information | addition of reducing agents has no effect; addition of reducing agents has no effect; addition of reducing agents has no effect | Catharanthus roseus | |
2.5.1.6 | putrescine | 15-25% inhibition with 5 mM; 15-25% inhibition with 5 mM; 15-25% inhibition with 5 mM | Catharanthus roseus | |
2.5.1.6 | spermidine | 15-34% inhibition with 5 mM; 15-34% inhibition with 5 mM; 15-34% inhibition with 5 mM | Catharanthus roseus | |
2.5.1.6 | spermine | 30-40% inhibition with 5 mM; 30-40% inhibition with 5 mM; 30-40% inhibition with 5 mM | Catharanthus roseus | |
2.5.1.6 | tripolyphosphate | strong inhibitor; strong inhibitor; strong inhibitor | Catharanthus roseus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.6 | 0.1 | - |
L-methionine | - |
Catharanthus roseus | |
2.5.1.6 | 0.18 | - |
ATP | - |
Catharanthus roseus | |
2.5.1.6 | 0.25 | - |
ATP | - |
Catharanthus roseus | |
2.5.1.6 | 0.31 | - |
ATP | - |
Catharanthus roseus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.6 | K+ | monovalent cations required for optimal activity, 50 mM K+ sufficient for full activity, can be replaced by NH4+ but not by Na+ | Catharanthus roseus | |
2.5.1.6 | Mg2+ | strictly dependent on divalent cations with maximum activity at 5mM and Mg2+ fully replaceable by Mn2+ or Co2+ salts | Catharanthus roseus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.6 | 42560 | - |
SAMS 3, gel filtration | Catharanthus roseus |
2.5.1.6 | 43000 | - |
SAMS 2, gel filtration | Catharanthus roseus |
2.5.1.6 | 43050 | - |
SAMS 1, gel filtration | Catharanthus roseus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.6 | Catharanthus roseus | Q96551 | SAMS1; Madagascar perwinkle, L. G. Don, line CP3a, three isoenzymes: SAMS 1, 2 and 3 | - |
2.5.1.6 | Catharanthus roseus | Q96552 | SAMS2; Madagascar perwinkle, L. G. Don, line CP3a, three isoenzymes: SAMS 1, 2 and 3 | - |
2.5.1.6 | Catharanthus roseus | Q96553 | SAMS3; Madagascar perwinkle, L. G. Don, line CP3a, three isoenzymes: SAMS 1, 2 and 3 | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.6 | ATP + L-methionine + H2O | - |
Catharanthus roseus | S-adenosyl-L-methionine + phosphate + diphosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.6 | monomer | gel filtration | Catharanthus roseus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.6 | 37 | - |
assay at | Catharanthus roseus |
2.5.1.6 | 37 | 45 | - |
Catharanthus roseus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.6 | 7 | 8.3 | three isoenzymes | Catharanthus roseus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.6 | 0.035 | - |
tripolyphosphate | - |
Catharanthus roseus |