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Literature summary extracted from

  • Shimamoto, S.; Moriyama, R.; Sugimoto, K.; Miyata, S.; Makino, S.
    Partial characterization of an enzyme fraction with protease activity which converts the spore peptidoglycan hydrolase (SleC) precursor to an active enzyme during germination of Clostridium perfringens S40 spores and analysis of a gene cluster involved in the activity (2001), J. Bacteriol., 183, 3742-3751.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.78 HgCl2 1 mM, 73% inhibition, addition of 10 mM dithiothreitol restores activity to 100% Clostridium perfringens
3.4.24.78 additional information not inhibited by E-64, pepstatin, bestatin, EDTA, antipain, APMSF, leupeptin, aprotinin and SSI Clostridium perfringens
3.4.24.78 p-chloromercuribenzenesulfonate 1 mM, 39% inhibition, addition of 10 mM dithiothreitol restores activity to 100% Clostridium perfringens
3.4.24.78 PMSF 1 mM, 68% inhibition after 1 h Clostridium perfringens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.78 51442
-
 x * 51442 + x * 55458 + x * 55585, deduced from nucleotide sequence Clostridium perfringens
3.4.24.78 52000
-
 x * 52000 + x * 57000 + x * 60000, processed forms of preproteins, SDS-PAGE Clostridium perfringens
3.4.24.78 55458
-
 x * 51442 + x * 55458 + x * 55585, deduced from nucleotide sequence Clostridium perfringens
3.4.24.78 55585
-
 x * 51442 + x * 55458 + x * 55585, deduced from nucleotide sequence Clostridium perfringens
3.4.24.78 57000
-
 x * 52000 + x * 57000 + x * 60000, processed forms of preproteins, SDS-PAGE Clostridium perfringens
3.4.24.78 60000
-
 x * 52000 + x * 57000 + x * 60000, processed forms of preproteins, SDS-PAGE Clostridium perfringens

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.78 Clostridium perfringens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.78 Superose 12, hydroxyapatite, DEAE-5PW Clostridium perfringens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.78 seed coat coat of dormant spores Clostridium perfringens
-
3.4.24.78 spore
-
 Clostridium perfringens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.78 additional information
-
 139363 units, 1 unit is defined as a decrease in the OD600 of 0.1 per min Clostridium perfringens

Subunits

EC Number Subunits Comment Organism
3.4.24.78 ? x * 52000 + x * 57000 + x * 60000, processed forms of preproteins, SDS-PAGE Clostridium perfringens
3.4.24.78 ? x * 51442 + x * 55458 + x * 55585, deduced from nucleotide sequence Clostridium perfringens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.78 4
-
 72 h, 80% loss of activity in the absence of CHAPS Clostridium perfringens
3.4.24.78 55
-
 40 min at pH 7.0, no loss of activity in the presence of 0.1% CHAPS Clostridium perfringens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.78 5.8 8.5
-
 Clostridium perfringens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.24.78 7
-
 at least 2 month at 4°C in the presence of 0.1% CHAPS, no loss of activity Clostridium perfringens