Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Eakin, A.E.; McGrath, M.E.; McKerrow, J.H.; Fletterick, R.J.; Craik, C.S.
    Production of crystallizable cruzain, the major cysteine protease from Trypanosoma cruzi (1993), J. Biol. Chem., 268, 6115-6118.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.51 enzyme and truncated version Trypanosoma cruzi

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.22.51 recombinant protein and truncated version Trypanosoma cruzi

Protein Variants

EC Number Protein Variants Comment Organism
3.4.22.51 C25A enzyme is inactive Trypanosoma cruzi
3.4.22.51 E219P variant with altered cleavage recognition site Trypanosoma cruzi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.51 0.0008
-
 benzyloxycarbonyl-Phe-Arg-aminomethyl coumarin truncated enzyme Trypanosoma cruzi
3.4.22.51 0.00096
-
 benzyloxycarbonyl-Phe-Arg-aminomethyl coumarin
-
 Trypanosoma cruzi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.51 23540
-
 electrospray mass spectrometry Trypanosoma cruzi

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.51 Trypanosoma cruzi Q598P5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.51
-
 Trypanosoma cruzi

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.51 epimastigote
-
 Trypanosoma cruzi
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.51 benzoyl-FR-4-methylcoumarin 7-amide + H2O
-
 Trypanosoma cruzi ?
-
 ?
3.4.22.51 benzoyl-YR-4-methylcoumarin 7-amide + H2O
-
 Trypanosoma cruzi ?
-
 ?
3.4.22.51 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O
-
 Trypanosoma cruzi ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.51 45
-
 E219P mutant, stable for 10 h Trypanosoma cruzi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.51 2.16
-
 benzyloxycarbonyl-Phe-Arg-aminomethyl coumarin truncated enzyme Trypanosoma cruzi
3.4.22.51 4.49
-
 benzyloxycarbonyl-Phe-Arg-aminomethyl coumarin
-
 Trypanosoma cruzi