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Literature summary extracted from

  • Cazzulo, J.J.; Cazzulo Franke, M.C.; Martinez, J.; Franke de Cazzulo, B.M.
    Some kinetic properties of a cysteine proteinase (cruzipain) from Trypanosoma cruzi (1990), Biochim. Biophys. Acta, 1037, 186-191.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.51 guanidine hydrochloride activates at low concentrations but inhibits above 2 M Trypanosoma cruzi
3.4.22.51 KSCN activates at low concentrations but inhibits above 1 M Trypanosoma cruzi
3.4.22.51 Urea maximal at 2 M with natural substrates, no activation with synthetic substrates Trypanosoma cruzi

General Stability

EC Number General Stability Organism
3.4.22.51 unstable in the absence of substrate at pH 8.7 and 37°C Trypanosoma cruzi

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.51 guanidine hydrochloride activates at low concentrations but inhibits above 2 M Trypanosoma cruzi
3.4.22.51 KSCN activates at low concentrations but inhibits above 1 M Trypanosoma cruzi
3.4.22.51 L-trans-epoxysuccinylleucylamido(4-guanidino)butane strong inhibitor Trypanosoma cruzi
3.4.22.51 Urea 70% inhibition at 5 M Trypanosoma cruzi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.51 0.011
-
 N-carbobenzoxy-Arg-Arg-2-(4-methoxy)-naphthylamide
-
 Trypanosoma cruzi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.51 35000 60000 SDS-PAGE, depending on electrophoretic conditions Trypanosoma cruzi
3.4.22.51 36300
-
 calculated from nucleotide sequence Trypanosoma cruzi

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.51 Trypanosoma cruzi Q598P5
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.51 epimastigote
-
 Trypanosoma cruzi
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.51 0.008 1.68 4-nitroanilide derivatives Trypanosoma cruzi
3.4.22.51 0.1 3.77 beta-naphthylamide derivatives Trypanosoma cruzi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.51 azocasein + H2O
-
 Trypanosoma cruzi ?
-
 ?
3.4.22.51 benzoyl-PFR-4-nitroanilide + H2O
-
 Trypanosoma cruzi ?
-
 ?
3.4.22.51 additional information substrates are chromogenic peptides with a carboxyl Arg or Lys, enzyme requires at least one more amino acid, preferably Arg, Phe, Val or Leu between the terminal Arg or Lys and the amino-blocking group Trypanosoma cruzi ?
-
 ?
3.4.22.51 N-carbobenzoxy-Arg-Arg-2-(4-methoxy)-naphthylamide + H2O
-
 Trypanosoma cruzi N-carbobenzoxy-Arg-Arg + 2-amino-4-methoxynaphthalene
-
 ?
3.4.22.51 peptidyl-4-nitroanilide + H2O derivatives of Trypanosoma cruzi ?
-
 ?
3.4.22.51 peptidyl-beta-naphthylamide + H2O derivatives of Trypanosoma cruzi ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.51 37
-
 unstable in the absence of substrate at pH 8.7 Trypanosoma cruzi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.51 0.0805
-
 N-carbobenzoxy-Arg-Arg-2-(4-methoxy)-naphthylamide
-
 Trypanosoma cruzi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.51 5
-
 assay with azocasein Trypanosoma cruzi
3.4.22.51 7
-
 beta-naphthylamide derivatives as substrate Trypanosoma cruzi
3.4.22.51 9
-
 4-nitroanilide derivatives as substrate Trypanosoma cruzi

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.22.51 8.7
-
 unstable in the absence of substrate at 37°C Trypanosoma cruzi