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Literature summary extracted from
- Expression in insect cells of active mature cruzipain from Trypanosoma cruzi, containing its C-terminal domain (2002), Protein Expr. Purif., 26, 467-475.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.22.51 | active complete mature enzyme | Trypanosoma cruzi |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.4.22.51 | - |
Trypanosoma cruzi |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.51 | 43000 | - |
deglycosylated enzyme, SDS-PAGE | Trypanosoma cruzi |
| 3.4.22.51 | 51000 | - |
glycosylated enzyme, SDS-PAGE | Trypanosoma cruzi |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.22.51 | Trypanosoma cruzi | Q598P5 | - |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.22.51 | glycoprotein | - |
Trypanosoma cruzi |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.22.51 | - |
Trypanosoma cruzi |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.51 | 84 | - |
- |
Trypanosoma cruzi |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.22.51 | additional information | synthetic chromogenic sustrates | Trypanosoma cruzi | ? | - |
? |  |
| 3.4.22.51 | peptidyl-4-nitroanilide + H2O | derivatives of | Trypanosoma cruzi | ? | - |
? | |
| 3.4.22.51 | peptidyl-beta-naphthylamide + H2O | derivatives of | Trypanosoma cruzi | ? | - |
? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.22.51 | additional information | - |
additional information | - |
Trypanosoma cruzi |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.51 | 6 | - |
assay with beta-naphthylamide | Trypanosoma cruzi |
| 3.4.22.51 | 7.6 | - |
assay with 4-nitroanilide | Trypanosoma cruzi |
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Release 2023.1 (January 2023)
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