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Literature summary extracted from

  • Bromme, D.; Okamoto, K.
    The baculovirus cysteine protease has a cathepsin B-like S2-subsite specificity (1995), Biol. Chem. Hoppe-Seyler, 376, 611-615.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.50 expression in Sf9 insect cells Autographa californica nucleopolyhedrovirus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.50 antipain 0.00005 mM, complete inhibition Autographa californica nucleopolyhedrovirus
3.4.22.50 chicken cystatin 0.0001 mM, complete inhibition Autographa californica nucleopolyhedrovirus
3.4.22.50 E64 0.0001 mM, complete inhibition Autographa californica nucleopolyhedrovirus
3.4.22.50 iodoacetate 0.05 mM, 80% inhibition Autographa californica nucleopolyhedrovirus
3.4.22.50 leupeptin 0.00005 mM, complete inhibition Autographa californica nucleopolyhedrovirus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.50 0.003
-
 Z-Arg-Arg-7-amido-4-methylcoumarin
-
 Autographa californica nucleopolyhedrovirus
3.4.22.50 0.022
-
 Z-Leu-Arg 7-amido-4-methylcoumarin
-
 Autographa californica nucleopolyhedrovirus
3.4.22.50 0.039
-
 Z-Phe-Arg 7-amido-4-methylcoumarin
-
 Autographa californica nucleopolyhedrovirus
3.4.22.50 0.043
-
 Z-Val-Arg-7-amido-4-methylcoumarin
-
 Autographa californica nucleopolyhedrovirus
3.4.22.50 0.058
-
 Z-Val-Val-Arg 7-amido-4-methylcoumarin
-
 Autographa californica nucleopolyhedrovirus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.50 Autographa californica nucleopolyhedrovirus P25783
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.50
-
 Autographa californica nucleopolyhedrovirus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.50 N-benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Autographa californica nucleopolyhedrovirus N-benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.50 N-benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O
-
 Autographa californica nucleopolyhedrovirus N-benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.50 N-benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Autographa californica nucleopolyhedrovirus N-benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.50 N-benzyloxycarbonyl-Val-Arg-7-amido-4-methylcoumarin + H2O
-
 Autographa californica nucleopolyhedrovirus N-benzyloxycarbonyl-Val-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.50 N-benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O
-
 Autographa californica nucleopolyhedrovirus N-benzyloxycarbonyl-Val-Val-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.50 Z-Leu-Arg 7-amido-4-methylcoumarin
-
 Autographa californica nucleopolyhedrovirus Z-Leu-Arg + 7-amino-4-methylcoumarin
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.50 0.51
-
 Z-Val-Val-Arg 7-amido-4-methylcoumarin
-
 Autographa californica nucleopolyhedrovirus
3.4.22.50 0.73
-
 Z-Arg-Arg-7-amido-4-methylcoumarin
-
 Autographa californica nucleopolyhedrovirus
3.4.22.50 0.73
-
 Z-Leu-Arg 7-amido-4-methylcoumarin
-
 Autographa californica nucleopolyhedrovirus
3.4.22.50 0.94
-
 Z-Val-Arg-7-amido-4-methylcoumarin
-
 Autographa californica nucleopolyhedrovirus
3.4.22.50 0.95
-
 Z-Phe-Arg 7-amido-4-methylcoumarin
-
 Autographa californica nucleopolyhedrovirus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.50 5 5.5 bell-shaped profile, hydrolysis of Z-Phe-Arg-7-amino-4-methylcoumarin and Z-Arg-Arg-7-amino-4-methylcoumarin Autographa californica nucleopolyhedrovirus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.50 3 7.5 hydrolysis of Z-Phe-Arg-7-amino-4-methylcoumarin Autographa californica nucleopolyhedrovirus
3.4.22.50 4 7.5 hydrolysis of Z-Arg-Arg-7-amino-4-methylcoumarin Autographa californica nucleopolyhedrovirus