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Literature summary extracted from

  • Fujimura, S.; Hirai, K.; Shibata, Y.; Nakayama, K.; Nakamura, T.
    Comparative properties of envelope-associated arginine-gingipains and lysine-gingipain of Porphyromonas gingivalis (1998), FEMS Microbiol. Lett., 163, 173-179.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.37 2-mercaptoethanol
-
 Porphyromonas gingivalis
3.4.22.37 cysteine
-
 Porphyromonas gingivalis
3.4.22.37 dithiothreitol
-
 Porphyromonas gingivalis
3.4.22.37 glycyl-glycine activates only RGP-B Porphyromonas gingivalis
3.4.22.47 2-mercaptoethanol 30 mM, 197% increase in activity Porphyromonas gingivalis
3.4.22.47 cysteine 3 mM, 166% increase in activity Porphyromonas gingivalis
3.4.22.47 dithiothreitol 30 mM, 315% increase in activity Porphyromonas gingivalis
3.4.22.47 EDTA 2 mM, 79% increase in activity Porphyromonas gingivalis
3.4.22.47 EGTA 2 mM, 79% increase in activity Porphyromonas gingivalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.37 antipain both enzyme forms Porphyromonas gingivalis
3.4.22.37 EDTA both enzyme forms Porphyromonas gingivalis
3.4.22.37 L-trans-epoxy-succinylleucylamido-(4-guanidino)butane both enzyme forms Porphyromonas gingivalis
3.4.22.37 leupeptin both enzyme forms Porphyromonas gingivalis
3.4.22.37 additional information no inhibition by aprotinin, epsilon-aminocaproic acid and diisopropylfluorophosphate Porphyromonas gingivalis
3.4.22.37 N-ethylmaleimide both enzyme forms Porphyromonas gingivalis
3.4.22.37 p-hydroxymercuribenzoate complete inhibition at 0.2 mM, both enzyme forms Porphyromonas gingivalis
3.4.22.37 tosyl-L-lysine chloromethyl ketone i.e. TLCK, both enzyme forms Porphyromonas gingivalis
3.4.22.47 N-ethylmaleimide 2 mM, 65% inhibition Porphyromonas gingivalis
3.4.22.47 p-hydroxymercuribenzoate 0.2 mM, 68% inhibition Porphyromonas gingivalis
3.4.22.47 tosyl-L-lysine chloromethyl ketone 0.05 mM, 81% inhibition Porphyromonas gingivalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.37 cell envelope
-
 Porphyromonas gingivalis 30313
-
3.4.22.37 extracellular
-
 Porphyromonas gingivalis
-
-
3.4.22.47 cell envelope
-
 Porphyromonas gingivalis 30313
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.22.37 additional information enzyme forms are not affected by Mg2+ and Ca2+ Porphyromonas gingivalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.37 43000
-
 x * 43000, both enzyme forms, SDS-PAGE Porphyromonas gingivalis
3.4.22.47 48000
-
 x * 48000, SDS-PAGE Porphyromonas gingivalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.37 protein + H2O Porphyromonas gingivalis
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.37 Porphyromonas gingivalis
-
 strain ATCC 33277, 2 arginine-specific gingipains RGP-A and RGP-B
-
3.4.22.47 Porphyromonas gingivalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.37 RGP-A and RGP-B, from envelope material, solubilization by detergent 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate, i.e. CHAPS Porphyromonas gingivalis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.47 culture supernatant
-
 Porphyromonas gingivalis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.37 benzoyl-L-arginine-4-nitroanilide + H2O
-
 Porphyromonas gingivalis benzoyl-L-arginine + 4-nitroaniline
-
 ?
3.4.22.37 benzoyl-Phe-Val-Arg-4-nitroanilide + H2O
-
 Porphyromonas gingivalis benzoyl-Phe-Val-Arg + 4-nitroaniline
-
 ?
3.4.22.37 benzoyl-Pro-Phe-Arg-4-nitroanilide + H2O
-
 Porphyromonas gingivalis benzoyl-Pro-Phe-Arg + 4-nitroaniline
-
 ?
3.4.22.37 benzoyl-Val-Gly-Arg-4-nitroanilide + H2O
-
 Porphyromonas gingivalis benzoyl-Val-Gly-Arg + 4-nitroaniline
-
 ?
3.4.22.37 butoxy-carbonyl-O-benzyl-Ser-Gly-Arg-4-nitroanilide + H2O best sythetic substrate Porphyromonas gingivalis butoxy-carbonyl-O-benzyl-Ser-Gly-Arg + 4-nitroaniline
-
 ?
3.4.22.37 butoxy-carbonyl-Val-Leu-Gly-Arg-4-nitroanilide + H2O
-
 Porphyromonas gingivalis butoxy-carbonyl-Val-Leu-Gly-Arg + 4-nitroaniline
-
 ?
3.4.22.37 haemoglobin + H2O complete digestion, enzyme form RGP-B shows high activity, not RGP-A, human substrate Porphyromonas gingivalis ?
-
 ?
3.4.22.37 additional information no activity of both enzyme forms with toluenesulfonyl-glycyl-L-prolyl-L-lysine-4-nitroanilide, Val-Leu-Lys-4-nitroanilide, benzoyl-Lys-4-nitroanilide, succinyl-Ala-Ala-Pro-Phe-4-nitroanilide, glutaryl-Phe-4-nitroanilide, benzoyl-Tyr-4-nitroanilide, and Lys-4-nitroanilide, activity with chromogenic synthetic substrates is limited to those with arginine in the P1-position Porphyromonas gingivalis ?
-
 ?
3.4.22.37 protein + H2O
-
 Porphyromonas gingivalis peptides
-
 ?
3.4.22.37 toluenesulfonyl-glycyl-L-prolyl-L-arginine-4-nitroanilide + H2O
-
 Porphyromonas gingivalis toluenesulfonyl-glycyl-L-prolyl-L-arginine + 4-nitroaniline
-
 ?
3.4.22.47 benzyloxycarbonyl-L-Lys-p-nitroanilide + H2O 7% of activity with N-p-tosyl-Gly-Pro-Lys-p-nitroanilide Porphyromonas gingivalis benzyloxycarbonyl-L-Lys + p-nitroaniline + H2O
-
 ?
3.4.22.47 D-Val-Phe-Lys-p-nitroanilide + H2O 70% of activity with N-p-tosyl-Gly-Pro-Lys-p-nitroanilide Porphyromonas gingivalis D-Val-Phe-Lys + p-nitroaniline
-
 ?
3.4.22.47 N-p-tosyl-Gly-Pro-Lys-p-nitroanilide + H2O
-
 Porphyromonas gingivalis N-p-tosyl-Gly-Pro-Lys + p-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.37 ? x * 43000, both enzyme forms, SDS-PAGE Porphyromonas gingivalis
3.4.22.47 ? x * 48000, SDS-PAGE Porphyromonas gingivalis

Synonyms

EC Number Synonyms Comment Organism
3.4.22.37 Arg-gingipain
-
 Porphyromonas gingivalis
3.4.22.37 arginine-gingipain
-
 Porphyromonas gingivalis
3.4.22.37 Arginine-specific gingipain
-
 Porphyromonas gingivalis
3.4.22.37 RGP
-
 Porphyromonas gingivalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.37 37
-
 assay at, substrate haemoglobin Porphyromonas gingivalis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.37 45
-
 50 mM Tris,-HCl, pH 7.8, 10 min, remaining activity for RGP-A is 82% and for RGP-B 52% of maximal activity, after 30 min remaining activity for RGP-A is 58% and for RGP-B 26% of maximal activity Porphyromonas gingivalis
3.4.22.47 45
-
 68% loss of activity after 10 min, 97% loss of activity after 30 min Porphyromonas gingivalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.37 7.5 8 both enzyme forms Porphyromonas gingivalis
3.4.22.47 7.5
-
-
 Porphyromonas gingivalis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.37 8.5
-
 rapid decrease of activity above pH 8.5 Porphyromonas gingivalis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.22.37 Porphyromonas gingivalis RGP-B, isoelectric focusing
-
 3.8
3.4.22.37 Porphyromonas gingivalis RGP-A, isoelectric focusing 4.8 4.7