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Literature summary extracted from

  • Burke, C.; Croteau, R.
    Interaction with the small subunit of geranyl diphosphate synthase modifies the chain length specificity of geranylgeranyl diphosphate synthase to produce geranyl diphosphate (2002), J. Biol. Chem., 277, 3141-3149.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.29 expression of preprotein and truncated versions in Escherichia coli Taxus canadensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.29 0.0026
-
 farnesyl diphosphate
-
 Taxus canadensis
2.5.1.29 0.0034
-
 Mg2+
-
 Taxus canadensis
2.5.1.29 0.0077
-
 geranyl diphosphate
-
 Taxus canadensis
2.5.1.29 0.039
-
 isopentenyl diphosphate cosubstrate farnesyl diphosphate Taxus canadensis
2.5.1.29 0.088
-
 isopentenyl diphosphate cosubstrate dimethylallyl diphosphate Taxus canadensis
2.5.1.29 0.122
-
 isopentenyl diphosphate cosubstrate geranyl diphosphate Taxus canadensis
2.5.1.29 0.127
-
 dimethylallyl diphosphate
-
 Taxus canadensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.29 32000
-
 2 * 32000, recombinant enzyme, SDS-PAGE Taxus canadensis
2.5.1.29 66000
-
 gel filtration Taxus canadensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.29 (E,E)-farnesyl diphosphate + isopentenyl diphosphate Abies grandis
-
 geranylgeranyl diphosphate + diphosphate
-
 ?
2.5.1.29 dimethylallyl diphosphate + 3 isopentenyl diphosphate Abies grandis
-
 geranylgeranyl diphosphate + 3 diphosphate
-
 ?
2.5.1.29 geranyl diphosphate + 2 isopentenyl diphosphate Abies grandis
-
 geranylgeranyl diphosphate + 2 diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.29 Abies grandis Q8W1R9
-
-
2.5.1.29 Taxus canadensis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.29 (E,E)-farnesyl diphosphate + isopentenyl diphosphate
-
 Abies grandis geranylgeranyl diphosphate + diphosphate
-
 ?
2.5.1.29 dimethylallyl diphosphate + 3 isopentenyl diphosphate
-
 Taxus canadensis geranylgeranyl diphosphate + 3 diphosphate
-
 ?
2.5.1.29 dimethylallyl diphosphate + 3 isopentenyl diphosphate
-
 Abies grandis geranylgeranyl diphosphate + 3 diphosphate
-
 ?
2.5.1.29 farnesyl diphosphate + isopentenyl diphosphate
-
 Taxus canadensis geranylgeranyl diphosphate + diphosphate
-
 ?
2.5.1.29 geranyl diphosphate + 2 isopentenyl diphosphate
-
 Abies grandis geranylgeranyl diphosphate + 2 diphosphate
-
 ?
2.5.1.29 geranyl diphosphate + isopentenyl diphosphate
-
 Taxus canadensis farnesyl diphosphate + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.29 dimer 2 * 32000, recombinant enzyme, SDS-PAGE Taxus canadensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.29 1
-
 dimethylallyl diphosphate
-
 Taxus canadensis
2.5.1.29 2.1
-
 geranyl diphosphate
-
 Taxus canadensis
2.5.1.29 3.3
-
 farnesyl diphosphate
-
 Taxus canadensis