EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.29 | expression in Saccharomyces cerevisiae | Taxus canadensis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.29 | 0.006 | - |
farnesyl diphosphate | recombinant enzyme | Taxus canadensis | |
2.5.1.29 | 0.007 | - |
isopentenyl diphosphate | recombinant enzyme | Taxus canadensis | |
2.5.1.29 | 0.035 | - |
Mg2+ | recombinant enzyme | Taxus canadensis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.29 | Mg2+ | required for activity | Taxus canadensis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.29 | 31500 | - |
2 * 31500, deduced from nucleotide sequence | Taxus canadensis |
2.5.1.29 | 60000 | - |
recombinant truncated enzyme, gel filtration | Taxus canadensis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.29 | (E,E)-farnesyl diphosphate + isopentenyl diphosphate | Taxus canadensis | 66% of activity with geranyl diphosphate | geranylgeranyl diphosphate + diphosphate | - |
? | |
2.5.1.29 | dimethylallyl diphosphate + 3 isopentenyl diphosphate | Taxus canadensis | 33% of activity with geranyl diphosphate | geranylgeranyl diphosphate + 3 diphosphate | - |
? | |
2.5.1.29 | geranyl diphosphate + 2 isopentenyl diphosphate | Taxus canadensis | preferred substrate | geranylgeranyl diphosphate + 2 diphosphate | - |
? | |
2.5.1.29 | geranyl diphosphate + 2 isopentenyl diphosphate | Taxus canadensis | involved in taxol biosynthesis | geranylgeranyl diphosphate + 2 diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.29 | Taxus canadensis | Q9ZPM3 | canadian yew | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.29 | recombinant enzyme | Taxus canadensis |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.5.1.29 | cell suspension culture | - |
Taxus canadensis | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.29 | (E,E)-farnesyl diphosphate + isopentenyl diphosphate | 66% of activity with geranyl diphosphate | Taxus canadensis | geranylgeranyl diphosphate + diphosphate | - |
? | |
2.5.1.29 | dimethylallyl diphosphate + 3 isopentenyl diphosphate | 33% of activity with geranyl diphosphate | Taxus canadensis | geranylgeranyl diphosphate + 3 diphosphate | - |
? | |
2.5.1.29 | geranyl diphosphate + 2 isopentenyl diphosphate | preferred substrate | Taxus canadensis | geranylgeranyl diphosphate + 2 diphosphate | - |
? | |
2.5.1.29 | geranyl diphosphate + 2 isopentenyl diphosphate | involved in taxol biosynthesis | Taxus canadensis | geranylgeranyl diphosphate + 2 diphosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.29 | dimer | 2 * 31500, deduced from nucleotide sequence | Taxus canadensis |