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Literature summary extracted from

  • Archer, B.L.; Cockbain, E.G.
    Rubber transferase from hevea brasiliensis latex (1969), Methods Enzymol., 15, 476-480.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.20 neryl diphosphate accelerates activity Hevea brasiliensis
2.5.1.20 sulfhydryl compound essential for full activity Hevea brasiliensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.20 EDTA 50 mM, strong inhibition Hevea brasiliensis
2.5.1.20 farnesyl diphosphate
-
 Hevea brasiliensis
2.5.1.20 iodoacetamide
-
 Hevea brasiliensis
2.5.1.20 N-ethylmaleimide
-
 Hevea brasiliensis
2.5.1.20 p-chloromercuribenzoate 50 mM, strong inhibition Hevea brasiliensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.20 Mg2+ magnesium compounds essential for full activity, cannot be replaced by manganese, cobalt or iron Hevea brasiliensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.20 60000
-
 gel filtration Hevea brasiliensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.20 isopentenyl diphosphate + cis-1,4-polyisoprene Hevea brasiliensis incorporation of isopentenyl diphosphate into rubber diphosphate + a poly-cis-polyprenyl diphosphate longer by one C5 unit
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.20 Hevea brasiliensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.20 pH 5.4, ammonium sulfate, Sephadex G-100, DEAE-Sephadex, partial purification Hevea brasiliensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.20 latex
-
 Hevea brasiliensis
-
2.5.1.20 leaf
-
 Hevea brasiliensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.20 isopentenyl diphosphate + cis-1,4-polyisoprene strictly stereospecific, yields exclusively cis-polyisoprene from isopentenyl diphosphate Hevea brasiliensis diphosphate + a poly-cis-polyprenyl diphosphate longer by one C5 unit
-
 ?
2.5.1.20 isopentenyl diphosphate + cis-1,4-polyisoprene of rubber particles Hevea brasiliensis diphosphate + a poly-cis-polyprenyl diphosphate longer by one C5 unit
-
 ?
2.5.1.20 isopentenyl diphosphate + cis-1,4-polyisoprene incorporation of isopentenyl diphosphate into rubber Hevea brasiliensis diphosphate + a poly-cis-polyprenyl diphosphate longer by one C5 unit
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.20 30
-
 assay at Hevea brasiliensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.20 40
-
 10 min, partial denaturation Hevea brasiliensis
2.5.1.20 60
-
 10 min, complete inactivation Hevea brasiliensis

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.20 additional information ascorbic acid is not cofactor Hevea brasiliensis