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Literature summary extracted from

  • Sagami, H.; Ogura, K.
    Geranylgeranyl pyrophosphate synthetase lacking geranyl-transferring activity from Micrococcus luteus (1981), J. Biochem., 89, 1573-1580.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.29 Triton X-100 stimulates farnesyl-transferring activity, inhibits dimethylallyl-transferring activity Micrococcus luteus
2.5.1.29 Tween 80 stimulates Micrococcus luteus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.29 diphosphate 10 mM, complete inhibition Micrococcus luteus
2.5.1.29 Triton X-100 stimulates farnesyl-transferring activity, inhibits dimethylallyl-transferring activity Micrococcus luteus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.29 0.008
-
 isopentenyl diphosphate cosubstrate dimethylallyl diphosphate at 0.175 mM Micrococcus luteus
2.5.1.29 0.062
-
 dimethylallyl diphosphate
-
 Micrococcus luteus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.29 Mg2+ required for activity Micrococcus luteus
2.5.1.29 Mg2+ optimal activation of farnesyl-transferring activity at 1 mM, optimal activation of dimethylallyl-transferring activity at 3 mM Micrococcus luteus
2.5.1.29 Mn2+ slight activation Micrococcus luteus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.29 70000
-
 gel filtration Micrococcus luteus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.29 (E,E)-farnesyl diphosphate + isopentenyl diphosphate Micrococcus luteus
-
 geranylgeranyl diphosphate + diphosphate
-
 ?
2.5.1.29 geranyl diphosphate + 2 isopentenyl diphosphate Micrococcus luteus
-
 geranylgeranyl diphosphate + 2 diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.29 Micrococcus luteus
-
 the activities of EC 2.5.1.1 and EC 2.5.1.29 may be a mixture of 2 enzymes or a single enzyme with two independent catalytic sites, see EC 2.5.1.1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.29 ammonium sulfate, DEAE-Sephadex, hydroxylapatite, Sephadex G-100, enzyme fraction may be a mixture of EC 2.5.1.1 and EC 2.5.1.29 or a single enzyme with two independent catalytic sites, see EC 2.5.1.1 Micrococcus luteus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.29 0.0182
-
-
 Micrococcus luteus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.29 (E)-3-methyl-2-dodecenyl diphosphate + isopentenyl diphosphate
-
 Micrococcus luteus (2E,5E)-3,6-dimethylpentadeca-2,5-dien-1-yl diphosphate + diphosphate
-
 ?
2.5.1.29 (E,E)-farnesyl diphosphate + isopentenyl diphosphate
-
 Micrococcus luteus geranylgeranyl diphosphate + diphosphate
-
 ?
2.5.1.29 dimethylallyl diphosphate + 3 isopentenyl diphosphate
-
 Micrococcus luteus geranylgeranyl diphosphate + 3 diphosphate
-
 ?
2.5.1.29 geranyl diphosphate + 2 isopentenyl diphosphate
-
 Micrococcus luteus geranylgeranyl diphosphate + 2 diphosphate
-
 ?
2.5.1.29 trans-3-methyl-2-octenyl diphosphate + isopentenyl diphosphate similar activity as with dimethylallyl diphosphate Micrococcus luteus ?
-
 ?
2.5.1.29 trans-3-methyl-2-pentenyl diphosphate + isopentenyl diphosphate 33% of activity with dimethylallyl diphosphate Micrococcus luteus ?
-
 ?