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Literature summary extracted from

  • Ogura, K.; Nishino, T.; Seto, S.
    The purification of prenyltransferase and isopentenyl pyrophosphate isomerase of pumpkin fruit and their some properties (1968), J. Biochem., 64, 197-203.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.10 iodoacetamide 5 mM, 31% inhibition Pumpkin

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.10 0.0013
-
 geranyl diphosphate
-
 Pumpkin

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.10 Mg2+ required for activity Pumpkin
2.5.1.10 Mn2+ can partially replace Mg2+, less effective Pumpkin

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.10 dimethylallyl diphosphate + 2 isopentenyl diphosphate Pumpkin enzyme participates in isoprenoid biosynthesis in eukaryotes (E,E)-farnesyl diphosphate + 2 diphosphate
-
 ?
2.5.1.10 isopentenyl diphosphate + geranyl diphosphate Pumpkin enzyme participates in isoprenoid biosynthesis in eukaryotes diphosphate + farnesyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.10 Pumpkin
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.10 ammonium sulfate, Sephadex G 200, hydroxylapatite Pumpkin

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.10 fruit
-
 Pumpkin
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.10 dimethylallyl diphosphate + 2 isopentenyl diphosphate activity is higher than with geranyl diphosphate Pumpkin (E,E)-farnesyl diphosphate + 2 diphosphate
-
 ?
2.5.1.10 dimethylallyl diphosphate + 2 isopentenyl diphosphate enzyme participates in isoprenoid biosynthesis in eukaryotes Pumpkin (E,E)-farnesyl diphosphate + 2 diphosphate
-
 ?
2.5.1.10 farnesyl diphosphate + isopentenyl diphosphate less than 5% of the activity with dimethylallyl diphosphate Pumpkin geranyl diphosphate + diphosphate
-
 ?
2.5.1.10 geranyl diphosphate + isopentenyl diphosphate
-
 Pumpkin diphosphate + farnesyl diphosphate
-
 ?
2.5.1.10 isopentenyl diphosphate + geranyl diphosphate enzyme participates in isoprenoid biosynthesis in eukaryotes Pumpkin diphosphate + farnesyl diphosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.10 7.5
-
-
 Pumpkin