EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.3.1.24 | additional information | chemically synthesized N-(5-phospho-beta-D-ribosyl)anthranilate contains inhibitors, but not if it is generated by anthranilate phosphoribosyltransferase | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.18 | 0.0016 | - |
anthranilate | - |
Saccharomyces cerevisiae | |
2.4.2.18 | 0.0224 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Saccharomyces cerevisiae | |
5.3.1.24 | additional information | - |
additional information | increasing the pH from 7.5 to 8.6 increases the Km | Escherichia coli | |
5.3.1.24 | 0.0049 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | pH 7.5 | Escherichia coli | |
5.3.1.24 | 0.0049 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | 25°C, bifunctional IGPS:PRAI | Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.2.18 | Mg2+ | dependent on | Saccharomyces cerevisiae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.2.18 | 42000 | - |
2 * 42000, SDS-PAGE | Saccharomyces cerevisiae |
2.4.2.18 | 83000 | - |
sedimentation equilibrium | Saccharomyces cerevisiae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.18 | anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | Saccharomyces cerevisiae | enzyme of tryptophan biosynthesis | N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | Escherichia coli | involved in tryptophan biosynthesis from chorismate | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | Saccharomyces cerevisiae | involved in tryptophan biosynthesis from chorismate | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.2.18 | Saccharomyces cerevisiae | - |
- |
- |
5.3.1.24 | Escherichia coli | - |
bifunctional indole-3-glycerol-phosphate synthetase/PRA isomerase complex | - |
5.3.1.24 | Saccharomyces cerevisiae | - |
naturally monofunctional enzyme | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.2.18 | 95% pure | Saccharomyces cerevisiae |
5.3.1.24 | - |
Saccharomyces cerevisiae |
5.3.1.24 | indole-3-glycerol-phosphate synthetase/PRA isomerase complex | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.2.18 | 1.58 | - |
- |
Saccharomyces cerevisiae |
EC Number | Storage Stability | Organism |
---|---|---|
2.4.2.18 | addition of 10% glycerol at -70°C and then dropping the solution into liquid N2, can be thawed and frozen several times without loss of activity | Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.18 | anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Saccharomyces cerevisiae | N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
2.4.2.18 | anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | enzyme of tryptophan biosynthesis | Saccharomyces cerevisiae | N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | - |
Escherichia coli | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | - |
Saccharomyces cerevisiae | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | involved in tryptophan biosynthesis from chorismate | Escherichia coli | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
5.3.1.24 | N-(5-phospho-beta-D-ribosyl)anthranilate | involved in tryptophan biosynthesis from chorismate | Saccharomyces cerevisiae | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.2.18 | dimer | 2 * 42000, SDS-PAGE | Saccharomyces cerevisiae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.2.18 | 25 | - |
assay at | Saccharomyces cerevisiae |
5.3.1.24 | 25 | - |
assay at | Escherichia coli |
5.3.1.24 | 25 | - |
assay at | Saccharomyces cerevisiae |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.2.18 | 4 | - |
loss of 10% activity in 24 h | Saccharomyces cerevisiae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.18 | 2.9 | - |
anthranilate | cosubstrate: 5-phospho-alpha-D-ribose 1-diphosphate | Saccharomyces cerevisiae | |
5.3.1.24 | 40 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | bifunctional IGPS:PRAI | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.2.18 | 7.5 | - |
assay at | Saccharomyces cerevisiae |
5.3.1.24 | 7.5 | - |
assay at | Escherichia coli |
5.3.1.24 | 7.5 | - |
assay at | Saccharomyces cerevisiae |