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Literature summary extracted from

  • Salerno, G.L.; Pontis, H.G.
    Sucrose phosphate synthetase, separation from sucrose synthetase and a study of its properties (1978), Planta, 142, 41-48.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.14 Mg2+ stimulates enzyme activity Triticum aestivum
2.4.1.14 Mn2+ stimulates enzyme activity Triticum aestivum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.14 ADP slight inhibition Triticum aestivum
2.4.1.14 ATP
-
 Triticum aestivum
2.4.1.14 citrate activity can by restored with Mg2+ Triticum aestivum
2.4.1.14 CTP
-
 Triticum aestivum
2.4.1.14 delta-gluconolactone
-
 Triticum aestivum
2.4.1.14 EDTA
-
 Triticum aestivum
2.4.1.14 GTP
-
 Triticum aestivum
2.4.1.14 Maleate
-
 Triticum aestivum
2.4.1.14 p-chloromercuribenzoate activity can be restored by addition of DTT or 2-mercaptoethanol Triticum aestivum
2.4.1.14 phosphate activity can by restored with Mg2+ Triticum aestivum
2.4.1.14 UDP competitive with UDP-glucose Triticum aestivum
2.4.1.14 UTP
-
 Triticum aestivum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.14 Mg2+ reverses inhibition with citrate, phosphate and nucloside triphosphates Triticum aestivum
2.4.1.14 Mn2+ stimulates enzyme activity Triticum aestivum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.14 380000 390000 gel filtration, glycerol density gradient centrifugation Triticum aestivum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.14 UDP-alpha-D-glucose + D-fructose 6-phosphate Triticum aestivum catalyzes the penultimate step of sucrose synthesis UDP + sucrose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.14 Triticum aestivum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.14 complete separation from sucrose synthetase Triticum aestivum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.14 germ
-
 Triticum aestivum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.14 0.68
-
 purified enzyme Triticum aestivum

Storage Stability

EC Number Storage Stability Organism
2.4.1.14 -20°C, 1 month, no loss of activity, 1 year, 50-60% loss of activity Triticum aestivum
2.4.1.14 0-4°C, stable in presence of 20% glycerol or 5 mM fructose-6-phosphate Triticum aestivum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.14 UDP-alpha-D-glucose + D-fructose 6-phosphate
-
 Triticum aestivum UDP + sucrose 6-phosphate
-
 ?
2.4.1.14 UDP-alpha-D-glucose + D-fructose 6-phosphate catalyzes the penultimate step of sucrose synthesis Triticum aestivum UDP + sucrose 6-phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.14 6.5
-
-
 Triticum aestivum