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Literature summary extracted from

  • Ruiz, A.; Winston, A.; Lim, Y.H.; Gilbert, B.A.; Rando, R.R.; Bok, D.
    Molecular and biochemical characterization of lecithin retinol acyltransferase (1999), J. Biol. Chem., 274, 3834-3841.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.135 expression in HEK-293 cells Homo sapiens
2.3.1.135 expression in HEK-293T cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.135 all-trans-retinyl-alpha-bromoacetate
-
 Bos taurus
2.3.1.135 N-Boc-L-biocytinyl-11-aminoundecan chloromethylketone 0.002 mM, 45% inhibition; irreversible inhibitor; time dependent inhibition Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.135 phosphatidylcholine + all-trans-retinol Bos taurus
-
 all-trans-retinyl acyl esters + 2-acylglycerophosphocholine
-
 ?
2.3.1.135 phosphatidylcholine + all-trans-retinol Homo sapiens
-
 all-trans-retinyl acyl esters + 2-acylglycerophosphocholine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.135 Bos taurus
-
-
-
2.3.1.135 Homo sapiens O95237
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.135 affinity labeling with all-trans-retinyl-alpha-bromoacetate, SDS electrophoresis, prior to affinity labelling, incubation with cholesterol chloroacetate to block nucleophilic reagents, or streptavidin column to remove endogenously biotinylated proteins enhance protein purification Bos taurus
2.3.1.135 affinity labeling with N-Boc-L-biocytinyl-11-aminoundecan chloromethylketone, precipitation and gel electrophoresis, prior to affinity labelling, incubation with cholesterol chloroacetate to block nucleophilic reagents, or streptavidin column to remove endogenously biotinylated proteins enhance protein purification Bos taurus
2.3.1.135 labeling with N-Boc-L-biocytinyl-11-aminoundecan chloromethylketone, affinity chromatography, avidin, prior to affinity chromatography or affinity labelling, incubation with cholesterol chloroacetate to block nucleophilic reagents, or streptavidin column to remove endogenously biotinylated proteins enhance protein purification Bos taurus
2.3.1.135 solubilization of membranes and centrifugation, chromatography on DEAE-column and Mono-Q column later on, quantitative information Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.135 liver mRNA expression in adult and fetal stage Homo sapiens
-
2.3.1.135 pancreas mRNA expression in adult stage Homo sapiens
-
2.3.1.135 retinal pigment epithelium
-
 Bos taurus
-
2.3.1.135 retinal pigment epithelium
-
 Homo sapiens
-
2.3.1.135 small intestine mRNA expression Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.135 dipalmitoylphosphatidylcholine + all-trans-retinol
-
 Bos taurus all-trans-retinyl palmitate + 2-palmitoylglycerophosphocholine
-
 r
2.3.1.135 dipalmitoylphosphatidylcholine + all-trans-retinol mechanistic hypothesis Homo sapiens all-trans-retinyl palmitate + 2-palmitoylglycerophosphocholine
-
 r
2.3.1.135 additional information
-
 Bos taurus ?
-
 ?
2.3.1.135 additional information only fatty acyl group at the sn-1 is transferred Homo sapiens ?
-
 ?
2.3.1.135 phosphatidylcholine + all-trans-retinol
-
 Bos taurus all-trans-retinyl acyl esters + 2-acylglycerophosphocholine
-
 ?
2.3.1.135 phosphatidylcholine + all-trans-retinol
-
 Homo sapiens all-trans-retinyl acyl esters + 2-acylglycerophosphocholine
-
 ?
2.3.1.135 phosphatidylcholine + all-trans-retinol-(bovine serum albumin)
-
 Homo sapiens all-trans-retinyl acyl ester-(bovine serum albumin) + 2-acylglycerophosphocholine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.135 monomer 1 × 25300, SDS-PAGE in presence of 2-mercaptoethanol, fully active catalytically Bos taurus
2.3.1.135 monomer 1 × 25000, fully active catalytically Homo sapiens