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Literature summary extracted from

  • Mitterbauer, R.; Karl, T.; Adam, G.
    Saccharomyces cerevisiae URH1 (encoding uridine-cytidine N-ribohydrolase): functional complementation by a nucleoside hydrolase from a protozoan parasite and by a mammalian uridine phosphorylase (2002), Appl. Environ. Microbiol., 68, 1336-1343.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.2.2.3 pharmacology Saccharomyces cerevisiae enzyme deficient mutant strains expressing protozoan enzymes could be useful tools in drug screening for specific inhibitors against the pathogenic parasites Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.3
-
 Saccharomyces cerevisiae
3.2.2.3 construction of enzyme overexpressing strains Saccharomyces cerevisiae
3.2.2.3 expression in a Saccharomyces mutant deficient in uridine-cytidine N-ribohydrolase restores the enzyme activity Homo sapiens
3.2.2.3 expression in a Saccharomyces mutant deficient in uridine-cytidine N-ribohydrolase restores the enzyme activity Crithidia fasciculata

Protein Variants

EC Number Protein Variants Comment Organism
3.2.2.3 additional information expression of uridine-cytidine N-ribohydrolase from protozoan parasite Crithidia fasciculata in a Saccharomyces mutant deficient restores the enzyme activity, as well as expression of the human enzyme in the yeast mutant Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.3 cytidine + H2O Homo sapiens
-
 D-ribose + cytosine
-
 ?
3.2.2.3 cytidine + H2O Saccharomyces cerevisiae
-
 D-ribose + cytosine
-
 ?
3.2.2.3 cytidine + H2O Crithidia fasciculata
-
 D-ribose + cytosine
-
 ?
3.2.2.3 additional information Saccharomyces cerevisiae key enzyme of the pyrimidine salvage pathway, overview, enzyme is required for growth of mutants deficient in uridine kinase activity ?
-
 ?
3.2.2.3 uridine + H2O Homo sapiens
-
 D-ribose + uracil
-
 ?
3.2.2.3 uridine + H2O Saccharomyces cerevisiae
-
 D-ribose + uracil
-
 ?
3.2.2.3 uridine + H2O Crithidia fasciculata
-
 D-ribose + uracil
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.3 Saccharomyces cerevisiae
-
 wild type and uridine phosphorylase deficient strain
-
3.2.2.3 Crithidia fasciculata Q27546 protozoan parasite
-
3.2.2.3 Homo sapiens
-
-
-
3.2.2.3 Saccharomyces cerevisiae
-
 enzyme URH1 or Urh1p, gene URH1, encoded in the ORF YDR400w
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.3 cytidine + phosphate
-
 Saccharomyces cerevisiae cytosine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.3 additional information no substrates: inosine, adenosine, guanosine, thymidine Saccharomyces cerevisiae ?
-
 ?
2.4.2.3 uridine + phosphate
-
 Saccharomyces cerevisiae uracil + alpha-D-ribose 1-phosphate
-
 ?
3.2.2.3 cytidine + H2O
-
 Homo sapiens D-ribose + cytosine
-
 ?
3.2.2.3 cytidine + H2O
-
 Saccharomyces cerevisiae D-ribose + cytosine
-
 ?
3.2.2.3 cytidine + H2O
-
 Crithidia fasciculata D-ribose + cytosine
-
 ?
3.2.2.3 additional information key enzyme of the pyrimidine salvage pathway, overview, enzyme is required for growth of mutants deficient in uridine kinase activity Saccharomyces cerevisiae ?
-
 ?
3.2.2.3 additional information inosine, adenosine, guanosine, and thymidine are poor substrates Saccharomyces cerevisiae ?
-
 ?
3.2.2.3 uridine + H2O
-
 Homo sapiens D-ribose + uracil
-
 ?
3.2.2.3 uridine + H2O
-
 Saccharomyces cerevisiae D-ribose + uracil
-
 ?
3.2.2.3 uridine + H2O
-
 Crithidia fasciculata D-ribose + uracil
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.2.3 URH1
-
 Saccharomyces cerevisiae
3.2.2.3 Urh1p
-
 Saccharomyces cerevisiae
3.2.2.3 uridine hydrolase
-
 Saccharomyces cerevisiae
3.2.2.3 uridine-cytidine N-ribohydrolase
-
 Homo sapiens
3.2.2.3 uridine-cytidine N-ribohydrolase
-
 Saccharomyces cerevisiae
3.2.2.3 uridine-cytidine N-ribohydrolase
-
 Crithidia fasciculata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.3 30
-
 assay at Saccharomyces cerevisiae