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Literature summary extracted from

  • Watanabe, S.; Takada, Y.; Fukunaga, N.
    Purification and characterization of a cold-adapted isocitrate lyase and a malate synthase from Colwellia maris, a psychrophilic bacterium (2001), Biosci. Biotechnol. Biochem., 65, 1095-1103.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.3.9 DTT and glycerol stabilize Colwellia maris
2.3.3.9 DTT stabilizes Colwellia maris
2.3.3.9 salt does not stabilize Colwellia maris

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.9 KCl
-
 Colwellia maris
2.3.3.9 NaCl
-
 Colwellia maris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.9 0.0198
-
 glyoxylate
-
 Colwellia maris
4.1.3.1 0.51
-
 DL-isocitrate 20°C, pH 6.8 Colwellia maris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.9 Co2+ about 35% of the activation with Mg2+ Colwellia maris
2.3.3.9 Mg2+ required Colwellia maris
2.3.3.9 Mn2+ 19% of the activation with Mg2+ Colwellia maris
2.3.3.9 Ni2+ about 98% of the activation with Mg2+ Colwellia maris
4.1.3.1 Co2+ can partly replace Mg2+ Colwellia maris
4.1.3.1 Mg2+ 10 mM, dependent Colwellia maris
4.1.3.1 Mn2+ can partly replace Mg2+ Colwellia maris
4.1.3.1 Ni2+ can partly replace Mg2+ Colwellia maris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.9 160000
-
 gel filtration Colwellia maris
2.3.3.9 763000
-
 2 * 763000, SDS-PAGE Colwellia maris
4.1.3.1 64700
-
 4 * 64700, SDS-PAGE Colwellia maris
4.1.3.1 240000
-
 gel filtration Colwellia maris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.9 glyoxylate + acetyl-CoA + H2O Colwellia maris enzyme specifically involved in glyoxalate cycle metabolism (S)-malate + CoA
-
 ?
2.3.3.9 glyoxylate + acetyl-CoA + H2O Colwellia maris enzyme essential for acetate use in the bacterial cells (S)-malate + CoA
-
 ?
4.1.3.1 isocitrate Colwellia maris
-
 succinate + glyoxylate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.9 Colwellia maris
-
 formerly Vibrio sp. strain ABE-1
-
4.1.3.1 Colwellia maris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.9
-
 Colwellia maris
4.1.3.1
-
 Colwellia maris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.3.9 culture condition:acetate-grown cell
-
 Colwellia maris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.9 69.6
-
-
 Colwellia maris
4.1.3.1 6.17
-
-
 Colwellia maris

Storage Stability

EC Number Storage Stability Organism
2.3.3.9 -20°C, stable for more than one month Colwellia maris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.9 glyoxylate + acetyl-CoA + H2O
-
 Colwellia maris (S)-malate + CoA
-
 ?
2.3.3.9 glyoxylate + acetyl-CoA + H2O enzyme specifically involved in glyoxalate cycle metabolism Colwellia maris (S)-malate + CoA
-
 ?
2.3.3.9 glyoxylate + acetyl-CoA + H2O enzyme essential for acetate use in the bacterial cells Colwellia maris (S)-malate + CoA
-
 ?
4.1.3.1 isocitrate
-
 Colwellia maris succinate + glyoxylate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.9 dimer 2 * 763000, SDS-PAGE Colwellia maris
4.1.3.1 tetramer 4 * 64700, SDS-PAGE Colwellia maris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.3.9 45
-
-
 Colwellia maris
4.1.3.1 20
-
-
 Colwellia maris

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.3.9 additional information
-
 Km for glyoxylate increases with decreasing temperature Colwellia maris
4.1.3.1 10
-
 half maximal enzyme activity Colwellia maris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.3.1 30
-
 enzyme is rapidly inactivated above, NaCl, 10% glycerol and 1 mM dithiothreitol protects Colwellia maris
4.1.3.1 35
-
 no enzyme activity Colwellia maris

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.3.9 60.7
-
 acetyl-CoA at 20°C Colwellia maris
2.3.3.9 161
-
 acetyl-CoA at 45°C Colwellia maris
4.1.3.1 18.1
-
 DL-isocitrate 20°C, pH 6.8 Colwellia maris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.9 8
-
-
 Colwellia maris
4.1.3.1 6.8
-
-
 Colwellia maris