EC Number | General Stability | Organism |
---|---|---|
2.4.2.4 | 2-mercaptoethanol and sucrose stabilize during preincubation procedure with ammonium sulfate | Salmonella enterica subsp. enterica serovar Typhimurium |
2.4.2.4 | quite labile during purification | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.4 | 1.3 | - |
arsenate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
2.4.2.4 | 2.1 | - |
thymidine | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
2.4.2.4 | 2.3 | - |
phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
2.4.2.4 | 8 | - |
deoxyuridine | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.2.4 | 47000 | - |
2 * 47000 | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.4 | thymine + 2-deoxy-D-ribose 1-phosphate | Salmonella enterica subsp. enterica serovar Typhimurium | in Escherichia coli and Salmonella typhimurium thymidine phosphorylase plays an important role in metabolism of thymine auxotrophs and is necessary for the conversion of exogenous thymine to thymidine | thymidine + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.2.4 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.2.4 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.2.4 | 465 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Storage Stability | Organism |
---|---|---|
2.4.2.4 | 4°C, 10 mM potassium phosphate, pH 7.5, 10 mM 2-mercaptoethanol, 20% sucrose, stable for 3 months | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.4 | bromodeoxyuridine + phosphate | at 40% the rate of thymidine | Salmonella enterica subsp. enterica serovar Typhimurium | bromouracil + 2-deoxy-D-ribose | - |
? | |
2.4.2.4 | deoxyuridine + phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | uracil + 2-deoxy-D-ribose 1-phosphate | - |
? | |
2.4.2.4 | iododeoxyuridine + phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | iodouracil + 2-deoxy-D-ribose | - |
? | |
2.4.2.4 | additional information | no substrate: deoxycytidine | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
2.4.2.4 | additional information | no substrates: deoxyadenosine, deoxyguanosine | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
2.4.2.4 | thymidine + arsenate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
2.4.2.4 | thymidine + phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | thymine + 2-deoxy-D-ribose 1-phosphate | - |
? | |
2.4.2.4 | thymine + 2-deoxy-D-ribose 1-phosphate | in Escherichia coli and Salmonella typhimurium thymidine phosphorylase plays an important role in metabolism of thymine auxotrophs and is necessary for the conversion of exogenous thymine to thymidine | Salmonella enterica subsp. enterica serovar Typhimurium | thymidine + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.2.4 | dimer | 2 * 47000 | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.2.4 | 37 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.2.4 | 7.4 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
2.4.2.4 | 7.5 | 8 | rapid decrease of activity above and below this range | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.4.2.4 | 7.5 | 8 | rapid decrease of activity above and below this range | Salmonella enterica subsp. enterica serovar Typhimurium |