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Literature summary extracted from

  • Entsch, B.; Parker, C.W.; Letham, D.S.
    An enzyme from lupin seeds forming alanine derivatives of cytokinins (1983), Phytochemistry, 22, 375-381.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.50 0.047
-
 O-acetyl-serine
-
 Lupinus luteus
2.5.1.50 0.88
-
 zeatin
-
 Lupinus luteus
2.5.1.50 2.5
-
 O-glycosylzeatin
-
 Lupinus luteus
2.5.1.50 26
-
 adenine
-
 Lupinus luteus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.50 64500
-
 gel filtration Lupinus luteus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.50 Lupinus luteus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.50 partial Lupinus luteus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.50 O-acetyl-L-serine + zeatin = lupinate + acetate ping pong bi bi mechanism Lupinus luteus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.50 seed
-
 Lupinus luteus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.50 0.009
-
-
 Lupinus luteus

Storage Stability

EC Number Storage Stability Organism
2.5.1.50 4°C, 2-3 months, stable Lupinus luteus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.50 O-acetyl-L-serine + 6-(2-hydroxyethylamino)purine 20% of the activity with zeatin Lupinus luteus 2-amino-3-(6-(2-hydroxyethyl)aminopurin-9yl)-propanoate + acetate
-
 ?
2.5.1.50 O-acetyl-L-serine + 6-(3,4-dimethoxybenzylamino)purine 7.5% of the activity with zeatin Lupinus luteus 2-amino-3-(6-(3,4-dimethoxybenzylamino)purin-9yl)-propanoate + acetate
-
 ?
2.5.1.50 O-acetyl-L-serine + 6-(6-hydroxyhexylamino)purine 64% of the activity with zeatin Lupinus luteus 2-amino-3-(6-aminohexylpurin-9yl)-propanoate + acetate
-
 ?
2.5.1.50 O-acetyl-L-serine + 6-(DELTA2-isopentenyl)aminopurine 290% of the activity with zeatin Lupinus luteus 2-amino-3-(6-(DELTA2-isopentenyl)aminopurin-9yl)-propanoate + acetate
-
 ?
2.5.1.50 O-acetyl-L-serine + 6-benzylaminopurine as active as zeatin Lupinus luteus 2-amino-3-(6-benzylaminopurin-9yl)-propanoate + acetate
-
 ?
2.5.1.50 O-acetyl-L-serine + 6-furfurylaminopurine 138% of the activity with zeatin Lupinus luteus 2-amino-3-(6-furfurylaminopurin-9yl)-propanoate + acetate
-
 ?
2.5.1.50 O-acetyl-L-serine + 6-isopentylaminopurine 99% of the activity with zeatin Lupinus luteus 2-amino-3-(6-isopentylaminopurin-9yl)-propanoate + acetate
-
 ?
2.5.1.50 O-acetyl-L-serine + 6-methylaminopurine 25.0% of the activity with zeatin Lupinus luteus 2-amino-3-(6-methylaminopurin-9-yl)propanoate + acetate
-
 ?
2.5.1.50 O-acetyl-L-serine + 6-propylaminopurine 57% of the activity with zeatin Lupinus luteus 2-amino-3-(6-propylaminopurin-9yl)-propanoate + acetate
-
 ?
2.5.1.50 O-acetyl-L-serine + adenine 7.5% of the activity with zeatin Lupinus luteus 2-amino-3-(6-aminopurin-9-yl)-propanoate + acetate
-
 ?
2.5.1.50 O-acetyl-L-serine + cis-zeatin 91% of the activity with zeatin Lupinus luteus lupinate + acetate
-
 ?
2.5.1.50 O-acetyl-L-serine + dihydrozeatin 30% of the activity with zeatin Lupinus luteus ?
-
 ?
2.5.1.50 O-acetyl-L-serine + O-beta-D-glucopyranosylzeatin 34% of the activity with zeatin Lupinus luteus ?
-
 ?
2.5.1.50 O-acetyl-L-serine + zeatin
-
 Lupinus luteus lupinate + acetate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.50 8
-
-
 Lupinus luteus