BRENDA - Enzyme Database

Characterization and membrane organization of beta 1-3- and beta 1-4-galactosyltransferases from human colonic adenocarcinoma cell lines Colo 205 and SW403: basis for preferential synthesis of type 1 chain lacto-series carbohydrate structures

Holmes, E.H.; Arch. Biochem. Biophys. 270, 630-646 (1989)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.179
Bovine liver non-specific transfer proteins
activation
Homo sapiens
2.4.1.179
CHAPSO
5 to 7fold less effective compared to Triton CF-54
Homo sapiens
2.4.1.179
deoxycholate
5 to 7fold less effective compared to Triton CF-54
Homo sapiens
2.4.1.179
additional information
little or no activation by phosphatidylcholine, phosphatidylglycerol, phosphatidylserine, phosphatidylinositol or cardiolipin, Brij-58, Empigen BB or detergent G-3634-A
Homo sapiens
2.4.1.179
phosphatidylethanolamine
activation, less efficient than Triton CF-54
Homo sapiens
2.4.1.179
taurodeoxycholate
5 to 7fold less effective compared to Triton CF-54
Homo sapiens
2.4.1.179
Triton CF-54
activation, optimal at 0.1%
Homo sapiens
2.4.1.179
Triton X-100
activation, can replace Triton CF-54 with 75% less efficiency
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.179
0.013
-
lactotriaosylceramide
-
Homo sapiens
2.4.1.179
0.048
-
UDP-galactose
-
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.179
endoplasmic reticulum
-
Homo sapiens
5783
-
2.4.1.179
Golgi apparatus
-
Homo sapiens
5794
-
2.4.1.179
membrane
-
Homo sapiens
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.179
Ca2+
activation, can replace Mn2+ with 26% efficiency
Homo sapiens
2.4.1.179
Co2+
activation, can replace Mn2+ with 51% efficiency
Homo sapiens
2.4.1.179
Cu2+
activation, can replace Mn2+ with 15% efficiency
Homo sapiens
2.4.1.179
Mg2+
activation, can replace Mn2+ with 13% efficiency
Homo sapiens
2.4.1.179
Mn2+
requirement, 25 mM
Homo sapiens
2.4.1.179
additional information
no activation by Zn2+ or Cd2+
Homo sapiens
2.4.1.179
Ni2+
activation, can replace Mn2+ with 16% efficiency
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.179
UDP-galactose + D-galactosyl-1,4-beta-D-glucosyl-glycolipid
Homo sapiens
associated with biosynthesis of type 1 lactoseries core chain carbohydrate structure
UDP + D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosyl-glycolipid
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.179
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.4.1.179
from Colo 205 cell line; partial
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.179
cell suspension culture
-
Homo sapiens
-
2.4.1.179
Colo-205 cell
colonic adenocarcino cell line
Homo sapiens
-
2.4.1.179
colonic adenocarcinoma cell
-
Homo sapiens
-
2.4.1.179
SW-403 cell
colonic adenocarcino cell line
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.1.179
0.00077
-
-
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.179
UDP-galactose + D-galactosyl-1,4-beta-D-glucosyl-glycolipid
associated with biosynthesis of type 1 lactoseries core chain carbohydrate structure
638380
Homo sapiens
UDP + D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosyl-glycolipid
-
-
-
-
2.4.1.179
UDP-galactose + D-galactosyl-1,4-beta-D-glucosyl-R
substrate specificity
638380
Homo sapiens
UDP + D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosyl-R
-
638380
Homo sapiens
?
2.4.1.179
UDP-galactose + lactotriaosylceramide
best substrate
638380
Homo sapiens
UDP + lactotetraosylceramide
-
638380
Homo sapiens
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.1.179
37
-
assay at
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.179
7
-
Hepes buffer
Homo sapiens
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.4.1.179
5.8
8.2
about half-maximal activity at pH 5.8 and pH 8.2, cacodylate or Tris-HCl buffer
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.179
Bovine liver non-specific transfer proteins
activation
Homo sapiens
2.4.1.179
CHAPSO
5 to 7fold less effective compared to Triton CF-54
Homo sapiens
2.4.1.179
deoxycholate
5 to 7fold less effective compared to Triton CF-54
Homo sapiens
2.4.1.179
additional information
little or no activation by phosphatidylcholine, phosphatidylglycerol, phosphatidylserine, phosphatidylinositol or cardiolipin, Brij-58, Empigen BB or detergent G-3634-A
Homo sapiens
2.4.1.179
phosphatidylethanolamine
activation, less efficient than Triton CF-54
Homo sapiens
2.4.1.179
taurodeoxycholate
5 to 7fold less effective compared to Triton CF-54
Homo sapiens
2.4.1.179
Triton CF-54
activation, optimal at 0.1%
Homo sapiens
2.4.1.179
Triton X-100
activation, can replace Triton CF-54 with 75% less efficiency
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.179
0.013
-
lactotriaosylceramide
-
Homo sapiens
2.4.1.179
0.048
-
UDP-galactose
-
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.179
endoplasmic reticulum
-
Homo sapiens
5783
-
2.4.1.179
Golgi apparatus
-
Homo sapiens
5794
-
2.4.1.179
membrane
-
Homo sapiens
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.179
Ca2+
activation, can replace Mn2+ with 26% efficiency
Homo sapiens
2.4.1.179
Co2+
activation, can replace Mn2+ with 51% efficiency
Homo sapiens
2.4.1.179
Cu2+
activation, can replace Mn2+ with 15% efficiency
Homo sapiens
2.4.1.179
Mg2+
activation, can replace Mn2+ with 13% efficiency
Homo sapiens
2.4.1.179
Mn2+
requirement, 25 mM
Homo sapiens
2.4.1.179
additional information
no activation by Zn2+ or Cd2+
Homo sapiens
2.4.1.179
Ni2+
activation, can replace Mn2+ with 16% efficiency
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.179
UDP-galactose + D-galactosyl-1,4-beta-D-glucosyl-glycolipid
Homo sapiens
associated with biosynthesis of type 1 lactoseries core chain carbohydrate structure
UDP + D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosyl-glycolipid
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.1.179
from Colo 205 cell line; partial
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.179
cell suspension culture
-
Homo sapiens
-
2.4.1.179
Colo-205 cell
colonic adenocarcino cell line
Homo sapiens
-
2.4.1.179
colonic adenocarcinoma cell
-
Homo sapiens
-
2.4.1.179
SW-403 cell
colonic adenocarcino cell line
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.1.179
0.00077
-
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.179
UDP-galactose + D-galactosyl-1,4-beta-D-glucosyl-glycolipid
associated with biosynthesis of type 1 lactoseries core chain carbohydrate structure
638380
Homo sapiens
UDP + D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosyl-glycolipid
-
-
-
-
2.4.1.179
UDP-galactose + D-galactosyl-1,4-beta-D-glucosyl-R
substrate specificity
638380
Homo sapiens
UDP + D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosyl-R
-
638380
Homo sapiens
?
2.4.1.179
UDP-galactose + lactotriaosylceramide
best substrate
638380
Homo sapiens
UDP + lactotetraosylceramide
-
638380
Homo sapiens
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.1.179
37
-
assay at
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.179
7
-
Hepes buffer
Homo sapiens
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.4.1.179
5.8
8.2
about half-maximal activity at pH 5.8 and pH 8.2, cacodylate or Tris-HCl buffer
Homo sapiens